Wilms Tumor 1b Expression Defines a Pro-regenerative Macrophage Subtype and Is Required for Organ Regeneration in the Zebrafish

Sanz-Morejon, Andrés; García-Redondo, Ana B.; Reuter, Hanna; Marques, Inês J.; Bates, Thomas; Galardi-Castilla, María; Große, Andreas; Manig, Steffi; Langa, Xavier; Ernst, Alexander; Piragyte, Indre; Botos, Marius-Alexandru; González-Rosa, Juan Manuel; Ruiz-Ortega, Marta; Briones, Ana M.; Salaices, Mercedes; Englert, Christoph; Mercader, Nadia (2019). Wilms Tumor 1b Expression Defines a Pro-regenerative Macrophage Subtype and Is Required for Organ Regeneration in the Zebrafish. Cell reports, 28(5), 1296-1306.e6. Cell Press 10.1016/j.celrep.2019.06.091

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Organ regeneration is preceded by the recruitment of innate immune cells, which play an active role during repair and regrowth. Here, we studied macrophage subtypes during organ regeneration in the zebrafish, an animal model with a high regenerative capacity. We identified a macrophage subpopulation expressing Wilms tumor 1b (wt1b), which accumulates within regenerating tissues. This wt1b+ macrophage population exhibited an overall pro-regenerative gene expression profile and different migratory behavior compared to the remainder of the macrophages. Functional studies showed that wt1b regulates macrophage migration and retention at the injury area. Furthermore, wt1b-null mutant zebrafish presented signs of impaired macrophage differentiation, delayed fin growth upon caudal fin amputation, and reduced cardiomyocyte proliferation following cardiac injury that correlated with altered macrophage recruitment to the regenerating areas. We describe a pro-regenerative macrophage subtype in the zebrafish and a role for wt1b in organ regeneration.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy > Functional Anatomy
09 Interdisciplinary Units > Microscopy Imaging Center (MIC)

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Sanz Morejon, Andrés; dos Santos Marques, Ines Joao; Ernst, Alexander Uwe Johann; Piragyté-Langa, Indré; Botos, Marius-Alexandru and Mercader Huber, Nadia Isabel

Subjects:

600 Technology > 610 Medicine & health
500 Science > 570 Life sciences; biology

ISSN:

2211-1247

Publisher:

Cell Press

Language:

English

Submitter:

Nadia Isabel Mercader Huber

Date Deposited:

20 Nov 2019 10:29

Last Modified:

20 Nov 2019 11:23

Publisher DOI:

10.1016/j.celrep.2019.06.091

PubMed ID:

31365871

BORIS DOI:

10.7892/boris.135135

URI:

https://boris.unibe.ch/id/eprint/135135

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