Sanz-Morejon, Andrés; García-Redondo, Ana B.; Reuter, Hanna; Marques, Inês J.; Bates, Thomas; Galardi-Castilla, María; Große, Andreas; Manig, Steffi; Langa, Xavier; Ernst, Alexander; Piragyte, Indre; Botos, Marius-Alexandru; González-Rosa, Juan Manuel; Ruiz-Ortega, Marta; Briones, Ana M.; Salaices, Mercedes; Englert, Christoph; Mercader, Nadia (2019). Wilms Tumor 1b Expression Defines a Pro-regenerative Macrophage Subtype and Is Required for Organ Regeneration in the Zebrafish. Cell reports, 28(5), 1296-1306.e6. Cell Press 10.1016/j.celrep.2019.06.091
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Organ regeneration is preceded by the recruitment of innate immune cells, which play an active role during repair and regrowth. Here, we studied macrophage subtypes during organ regeneration in the zebrafish, an animal model with a high regenerative capacity. We identified a macrophage subpopulation expressing Wilms tumor 1b (wt1b), which accumulates within regenerating tissues. This wt1b+ macrophage population exhibited an overall pro-regenerative gene expression profile and different migratory behavior compared to the remainder of the macrophages. Functional studies showed that wt1b regulates macrophage migration and retention at the injury area. Furthermore, wt1b-null mutant zebrafish presented signs of impaired macrophage differentiation, delayed fin growth upon caudal fin amputation, and reduced cardiomyocyte proliferation following cardiac injury that correlated with altered macrophage recruitment to the regenerating areas. We describe a pro-regenerative macrophage subtype in the zebrafish and a role for wt1b in organ regeneration.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy 04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy > Functional Anatomy 09 Interdisciplinary Units > Microscopy Imaging Center (MIC) |
Graduate School: |
Graduate School for Cellular and Biomedical Sciences (GCB) |
UniBE Contributor: |
Sanz Morejon, Andrés, dos Santos Marques, Ines Joao, Ernst, Alexander Uwe Johann, Piragyté-Langa, Indré, Botos Rogozsan, Marius-Alexandru, Mercader Huber, Nadia Isabel |
Subjects: |
600 Technology > 610 Medicine & health 500 Science > 570 Life sciences; biology |
ISSN: |
2211-1247 |
Publisher: |
Cell Press |
Language: |
English |
Submitter: |
Nadia Isabel Mercader Huber |
Date Deposited: |
20 Nov 2019 10:29 |
Last Modified: |
05 Dec 2022 15:32 |
Publisher DOI: |
10.1016/j.celrep.2019.06.091 |
PubMed ID: |
31365871 |
BORIS DOI: |
10.7892/boris.135135 |
URI: |
https://boris.unibe.ch/id/eprint/135135 |
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