Metabolomics by UHPLC-MS: benefits provided by complementary use of Q-TOF and QQQ for pathway profiling.

Freiburghaus, Katrin; Largiadèr, Carlo Rodolfo; Stettler, Christoph; Fiedler, Georg Martin; Bally, Lia; Bovet, Cédric (2019). Metabolomics by UHPLC-MS: benefits provided by complementary use of Q-TOF and QQQ for pathway profiling. Metabolomics, 15(9), p. 120. Springer 10.1007/s11306-019-1585-3

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INTRODUCTION Non-targeted metabolic profiling using high-resolution mass spectrometry (HRMS) is a standard approach for pathway identification despite technical limitations. OBJECTIVES To assess the performance of combining targeted quadrupole (QQQ) analysis with HRMS for in-depth pathway profiling. METHODS Serum of exercising patients with type 1 diabetes (T1D) was profiled using targeted and non-targeted assays. RESULTS Non-targeted analysis yielded a broad unbiased metabolic profile, targeted analysis increased coverage of purine metabolism (twofold) and TCA cycle (three metabolites). CONCLUSION Our screening strategy combined the benefits of the unbiased full-scan HRMS acquisition with the deeper insight into specific pathways by large-scale QQQ analysis.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Gynaecology, Paediatrics and Endocrinology (DFKE) > Clinic of Endocrinology, Diabetology and Clinical Nutrition
04 Faculty of Medicine > Department of Haematology, Oncology, Infectious Diseases, Laboratory Medicine and Hospital Pharmacy (DOLS) > Institute of Clinical Chemistry

UniBE Contributor:

Freiburghaus, Katrin; Largiadèr, Carlo Rodolfo; Stettler, Christoph; Fiedler, Georg Martin; Bally, Lia Claudia and Bovet, Cédric

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1573-3882

Publisher:

Springer

Language:

English

Submitter:

Alexandra Müller

Date Deposited:

30 Dec 2019 14:02

Last Modified:

05 Jan 2020 02:44

Publisher DOI:

10.1007/s11306-019-1585-3

PubMed ID:

31463683

Uncontrolled Keywords:

Metabolism Non-targeted Pathways Targeted

BORIS DOI:

10.7892/boris.136877

URI:

https://boris.unibe.ch/id/eprint/136877

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