Freiburghaus, Katrin; Largiadèr, Carlo Rodolfo; Stettler, Christoph; Fiedler, Georg Martin; Bally, Lia; Bovet, Cédric (2019). Metabolomics by UHPLC-MS: benefits provided by complementary use of Q-TOF and QQQ for pathway profiling. Metabolomics, 15(9), p. 120. Springer 10.1007/s11306-019-1585-3
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INTRODUCTION
Non-targeted metabolic profiling using high-resolution mass spectrometry (HRMS) is a standard approach for pathway identification despite technical limitations.
OBJECTIVES
To assess the performance of combining targeted quadrupole (QQQ) analysis with HRMS for in-depth pathway profiling.
METHODS
Serum of exercising patients with type 1 diabetes (T1D) was profiled using targeted and non-targeted assays.
RESULTS
Non-targeted analysis yielded a broad unbiased metabolic profile, targeted analysis increased coverage of purine metabolism (twofold) and TCA cycle (three metabolites).
CONCLUSION
Our screening strategy combined the benefits of the unbiased full-scan HRMS acquisition with the deeper insight into specific pathways by large-scale QQQ analysis.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
04 Faculty of Medicine > Department of Gynaecology, Paediatrics and Endocrinology (DFKE) > Clinic of Endocrinology, Diabetology and Clinical Nutrition 04 Faculty of Medicine > Department of Haematology, Oncology, Infectious Diseases, Laboratory Medicine and Hospital Pharmacy (DOLS) > Institute of Clinical Chemistry |
UniBE Contributor: |
Freiburghaus, Katrin, Largiadèr, Carlo Rodolfo, Stettler, Christoph, Fiedler, Georg Martin, Bally, Lia Claudia, Bovet, Cédric |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
1573-3882 |
Publisher: |
Springer |
Language: |
English |
Submitter: |
Alexandra Müller |
Date Deposited: |
30 Dec 2019 14:02 |
Last Modified: |
30 Aug 2023 00:25 |
Publisher DOI: |
10.1007/s11306-019-1585-3 |
PubMed ID: |
31463683 |
Uncontrolled Keywords: |
Metabolism Non-targeted Pathways Targeted |
BORIS DOI: |
10.7892/boris.136877 |
URI: |
https://boris.unibe.ch/id/eprint/136877 |
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