rRNA expansion segment 27Lb modulates the factor recruitment capacity of the yeast ribosome and shapes the proteome.

Shankar, Vaishnavi; Rauscher, Robert; Reuther, Julia; Gharib, Walid H; Koch, Miriam; Polacek, Norbert (2020). rRNA expansion segment 27Lb modulates the factor recruitment capacity of the yeast ribosome and shapes the proteome. Nucleic acids research, 48(6), pp. 3244-3256. Oxford University Press 10.1093/nar/gkaa003

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Fine-tuned regulation of protein biosynthesis is crucial for cellular fitness and became even more vital when cellular and organismal complexity increased during the course of evolution. In order to cope with this augmented demand for translation control, eukaryal ribosomes have gained extensions both at the ribosomal protein and rRNA levels. Here we analyze the functional role of ES27L, an rRNA expansion segment in the large ribosomal subunit of Saccharomyces cerevisiae. Deletion of the b-arm of this expansion segment, called ES27Lb, did not hamper growth during optimal conditions, thus demonstrating that this 25S rRNA segment is not inherently crucial for ribosome functioning. However, reductive stress results in retarded growth and rendered unique protein sets prone to aggregation. Lack of ES27Lb negatively affects ribosome-association of known co-translational N-terminal processing enzymes which in turn contributes to the observed protein aggregation. Likely as a compensatory response to these challenges, the truncated ribosomes showed re-adjusted translation of specific sets of mRNAs and thus fine-tune the translatome in order to re-establish proteostasis. Our study gives comprehensive insight into how a highly conserved eukaryal rRNA expansion segment defines ribosomal integrity, co-translational protein maturation events and consequently cellular fitness.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Departement of Chemistry and Biochemistry

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Ganapathy Shankar, Vaishnavi; Rauscher, Robert; Reuther, Julia; Gharib, Walid and Polacek, Norbert

Subjects:

500 Science > 570 Life sciences; biology
500 Science > 540 Chemistry

ISSN:

0305-1048

Publisher:

Oxford University Press

Language:

English

Submitter:

Andrea Stettler

Date Deposited:

31 Jan 2020 17:09

Last Modified:

31 Mar 2020 01:33

Publisher DOI:

10.1093/nar/gkaa003

PubMed ID:

31960048

BORIS DOI:

10.7892/boris.139713

URI:

https://boris.unibe.ch/id/eprint/139713

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