Nitroreductases of bacterial origin in Giardia lamblia : Potential role in detoxification of xenobiotics

Müller, Joachim; Müller, Norbert (2019). Nitroreductases of bacterial origin in Giardia lamblia : Potential role in detoxification of xenobiotics. MicrobiologyOpen, 8(10), pp. 1-9. Wiley 10.1002/mbo3.904

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The anaerobic parasite Giardia lamblia, causative agent of persistent diarrhea, contains a family of nitroreductase genes most likely acquired by lateral transfer from anaerobic bacteria or archaebacteria. Two of these nitroreductases, containing a ferredoxin domain at their N-terminus, NR1, and NR2, have been characterized previously. Here, we present the characterization of a third member of this family, NR3. In functional assays, recombinant NR1 and NR3 reduced quinones like menadione and the antibiotic tetracycline, and-to much lesser extents-the nitro compound dinitrotoluene. Conversely, recombinant NR2 had no activity on tetracycline. Escherichia coli expressing NR3 were less susceptible to tetracycline, but more susceptible to the nitro compound metronidazole under semi-aerobic growth conditions. G. lamblia overexpressing NR1 and NR3, but not lines overexpressing NR2, are more susceptible to the nitro drug nitazoxanide. These findings suggest that NR3 is an active quinone reductase with a mode of action similar to NR1, but different from NR2. The biological function of this family of enzymes may reside in the use of xenobiotics as final electron acceptors. Thereby, these enzymes may provide at least two evolutionary advantages namely a higher potential to recycle NAD(P) as electron acceptors for the (fermentative) energy and intermediary metabolism, and the possibility to inactivate toxic xenobiotics produced by microorganisms living in concurrence inside the intestinal habitat.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Parasitology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)

UniBE Contributor:

Müller, Joachim and Müller, Norbert

Subjects:

600 Technology > 630 Agriculture
500 Science > 570 Life sciences; biology
500 Science > 590 Animals (Zoology)

ISSN:

2045-8827

Publisher:

Wiley

Funders:

[42] Schweizerischer Nationalfonds

Projects:

Projects 0 not found.

Language:

English

Submitter:

Norbert Müller

Date Deposited:

27 Mar 2020 12:00

Last Modified:

27 Mar 2020 12:09

Publisher DOI:

10.1002/mbo3.904

PubMed ID:

31343119

BORIS DOI:

10.7892/boris.141924

URI:

https://boris.unibe.ch/id/eprint/141924

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