COMPARISON OF IN VITRO PRE-ECLAMPSIA (PE) CELL MODELS WITH PE PLACENTAL TISSUE

Kallol, Sampada; Briggs, Louise; Mistry, Hiten D.; Moser, Ruedi; Schneider, Henning; Albrecht, Christiane (2019). COMPARISON OF IN VITRO PRE-ECLAMPSIA (PE) CELL MODELS WITH PE PLACENTAL TISSUE. Placenta, 83, e104-e104. Elsevier

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Objectives: We induced hypoxia and oxidative stress (OS) in cytotrophoblasts
(CTB) from control placentae to mimic the characteristics of preeclampsia
(PE). For validation we compared the gene and protein
expression of established PE biomarkers between the cell models and PE
placentae.
Methods: CTB isolated from healthy term placentae (n¼2) were cultured
either under normoxic (5% pCO2;21% pO2;24h), hypoxic (5% pCO2;1.5%
pO2;24h) or OS (consecutive 6h-intervals of normoxia and hypoxia until
24h) conditions. mRNA abundances of PE biomarkers (Table1) were analysed
by qRT-PCR in the cell models and PE placentae (n¼12). Additionally,
the secretion of sFlt1 and PlGF was determined by ELISA in cell culture
supernatants. Statistical analysis was performed using unpaired t-test.
Results: The cell models recapitulated important pathophysiological
changes occurring in PE (Table1) including the upregulation of sFlt1 which
was also observed in PE placentae. Interestingly, in the cell models the
sFlt1/PlGF ratio was above the clinically applied cutoff value of 38 for
hypoxic (77.3±35.2) and OS (124±88.3), but <38 for normoxic (19.4±16)
conditions.
nm-not measured
Conclusion: The established cell models are mimicking important characteristics
of PE. The sFlt1/PlGF ratio obtained in cell models is in agreement
with reports proposing sFlt1/PlGF as predictive diagnostic marker for
PE. Thus, hypoxia and OS induced in primary CTB can serve as suitable
models to study the detailed mechanisms of PE.

Item Type:

Conference or Workshop Item (Abstract)

Division/Institute:

04 Faculty of Medicine > Faculty Institutions > NCCR TransCure
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Biochemistry and Molecular Medicine

UniBE Contributor:

Kallol, Sampada Arvindrao and Albrecht, Christiane

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

0143-4004

Publisher:

Elsevier

Language:

English

Submitter:

Barbara Järmann-Bangerter

Date Deposited:

02 Jul 2020 17:14

Last Modified:

10 Aug 2020 18:50

BORIS DOI:

10.7892/boris.144221

URI:

https://boris.unibe.ch/id/eprint/144221

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