Rapid Quantification of SARS-CoV-2-Neutralizing Antibodies Using Propagation-Defective Vesicular Stomatitis Virus Pseudotypes.

Zettl, Ferdinand; Meister, Toni Luise; Vollmer, Tanja; Fischer, Bastian; Steinmann, Jörg; Krawczyk, Adalbert; V'kovski, Philip; Todt, Daniel; Steinmann, Eike; Pfaender, Stephanie; Zimmer, Gert (2020). Rapid Quantification of SARS-CoV-2-Neutralizing Antibodies Using Propagation-Defective Vesicular Stomatitis Virus Pseudotypes. Vaccines, 8(3) MDPI 10.3390/vaccines8030386

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Severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2, a new member of the genus Betacoronavirus, is a pandemic virus, which has caused numerous fatalities, particularly in the elderly and persons with underlying morbidities. At present, there are no approved vaccines nor antiviral therapies available. The detection and quantification of SARS-CoV-2-neutralizing antibodies plays a crucial role in the assessment of the immune status of convalescent COVID-19 patients, evaluation of recombinant therapeutic antibodies, and the evaluation of novel vaccines. To detect SARS-CoV-2-neutralizing antibodies, classically, a virus-neutralization test has to be performed at biosafety level 3, considerably limiting the general use of this test. In the present work, a biosafety level 1 pseudotype virus assay based on a propagation-incompetent vesicular stomatitis virus (VSV) has been used to determine the neutralizing antibody titers in convalescent COVID-19 patients. The neutralization titers in serum of two independently analyzed patient cohorts were available within 18 h and correlated well with those obtained with a classical SARS-CoV-2 neutralization test (Pearson correlation coefficients of r = 0.929 and r = 0.939, respectively). Most convalescent COVID-19 patients had only low titers of neutralizing antibodies (ND50 < 320). The sera of convalescent COVID-19 patients also neutralized pseudotype virus displaying the SARS-CoV-1 spike protein on their surface, which is homologous to the SARS-CoV-2 spike protein. In summary, we report a robust virus-neutralization assay, which can be used at low biosafety level 1 to rapidly quantify SARS-CoV-2-neutralizing antibodies in convalescent COVID-19 patients and vaccinated individuals.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Virology and Immunology

UniBE Contributor:

V'kovski, Philip and Zimmer, Gert

Subjects:

600 Technology > 630 Agriculture

ISSN:

2076-393X

Publisher:

MDPI

Language:

English

Submitter:

Andrea Stettler

Date Deposited:

04 Aug 2020 14:57

Last Modified:

04 Aug 2020 15:01

Publisher DOI:

10.3390/vaccines8030386

PubMed ID:

32679691

Uncontrolled Keywords:

SARS-CoV-2 neutralizing antibodies passive immunization pseudovirus vaccine

BORIS DOI:

10.7892/boris.145564

URI:

https://boris.unibe.ch/id/eprint/145564

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