Parainfluenza Virus 5 Infection in Neurological Disease and Encephalitis of Cattle.

Hierweger, Melanie M.; Werder, Simea; Seuberlich, Torsten (2020). Parainfluenza Virus 5 Infection in Neurological Disease and Encephalitis of Cattle. International journal of molecular sciences, 21(2) Molecular Diversity Preservation International MDPI 10.3390/ijms21020498

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The etiology of viral encephalitis in cattle often remains unresolved, posing a potential risk for animal and human health. In metagenomics studies of cattle with bovine non-suppurative encephalitis, parainfluenza virus 5 (PIV5) was identified in three brain samples. Interestingly, in two of these animals, bovine herpesvirus 6 and bovine astrovirus CH13 were additionally found. We investigated the role of PIV5 in bovine non-suppurative encephalitis and further characterized the three cases. With traditional sequencing methods, we completed the three PIV5 genomes, which were compared to one another. However, in comparison to already described PIV5 strains, unique features were revealed, like an 81 nucleotide longer open reading frame encoding the small hydrophobic (SH) protein. With in situ techniques, we demonstrated PIV5 antigen and RNA in one animal and found a broad cell tropism of PIV5 in the brain. Comparative quantitative analyses revealed a high viral load of PIV5 in the in situ positive animal and therefore, we propose that PIV5 was probably the cause of the disease. With this study, we clearly show that PIV5 is capable of naturally infecting different brain cell types in cattle in vivo and therefore it is a probable cause of encephalitis and neurological disease in cattle.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Experimental Clinical Research
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH)

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Hierweger, Melanie Michaela; Werder, Simea and Seuberlich, Torsten

Subjects:

500 Science
500 Science > 540 Chemistry
500 Science > 570 Life sciences; biology
500 Science > 590 Animals (Zoology)
600 Technology > 630 Agriculture

ISSN:

1661-6596

Publisher:

Molecular Diversity Preservation International MDPI

Language:

English

Submitter:

Melanie Michaela Hierweger

Date Deposited:

14 Aug 2020 14:41

Last Modified:

14 Aug 2020 14:49

Publisher DOI:

10.3390/ijms21020498

PubMed ID:

31941046

Uncontrolled Keywords:

bovine astrovirus bovine herpesvirus cattle encephalitis genome analysis in situ hybridization neurological disease parainfluenza virus 5 simian virus 5 virus discovery

BORIS DOI:

10.7892/boris.145788

URI:

https://boris.unibe.ch/id/eprint/145788

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