Peichel, Catherine L.; McCann, Shaugnessy R; Ross, Joseph A; Naftaly, Alice FS; Urton, James R; Cech, Jennifer N; Grimwood, Jane; Schmutz, Jeremy; Myers, Richard M; Kingsley, David M; White, Michael A (2020). Assembly of the threespine stickleback Y chromosome reveals convergent signatures of sex chromosome evolution. Genome biology, 21(1), p. 177. BioMed Central Ltd. 10.1186/s13059-020-02097-x
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Background: Heteromorphic sex chromosomes have evolved repeatedly across diverse species. Suppression of recombination between X and Y chromosomes leads to degeneration of the Y chromosome. The progression of degeneration is not well understood, as complete sequence assemblies of heteromorphic Y chromosomes have only been generated across a handful of taxa with highly degenerate sex chromosomes. Here, we describe the assembly of the threespine stickleback (Gasterosteus aculeatus) Y chromosome, which is less than 26 million years old and at an intermediate stage of degeneration. Our previous work identified that the nonrecombining region between the X and the Y spans approximately 17.5 Mb on the X chromosome.
Results: We combine long-read sequencing with a Hi-C-based proximity guided
assembly to generate a 15.87 Mb assembly of the Y chromosome. Our assembly is
concordant with cytogenetic maps and Sanger sequences of over 90 Y chromosome BAC clones. We find three evolutionary strata on the Y chromosome, consistent with the three inversions identified by our previous cytogenetic analyses. The threespine stickleback Y shows convergence with more degenerate sex chromosomes in the retention of haploinsufficient genes and the accumulation of genes with testis biased expression, many of which are recent duplicates. However, we find no evidence for large amplicons identified in other sex chromosome systems. We also report an excellent candidate for the master sex-determination gene: a translocated copy of Amh (Amhy).
Conclusions: Together, our work shows that the evolutionary forces shaping sex
chromosomes can cause relatively rapid changes in the overall genetic architecture of Y chromosomes.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
09 Interdisciplinary Units > Next Generation Sequencing (NGS) Platform 08 Faculty of Science > Department of Biology > Institute of Ecology and Evolution (IEE) > Evolutionary Ecology |
UniBE Contributor: |
Peichel, Catherine |
Subjects: |
500 Science > 570 Life sciences; biology |
ISSN: |
1465-6906 |
Publisher: |
BioMed Central Ltd. |
Funders: |
[4] Swiss National Science Foundation |
Language: |
English |
Submitter: |
Catherine Peichel |
Date Deposited: |
07 Sep 2020 14:06 |
Last Modified: |
31 Dec 2022 07:10 |
Publisher DOI: |
10.1186/s13059-020-02097-x |
PubMed ID: |
32684159 |
BORIS DOI: |
10.7892/boris.146369 |
URI: |
https://boris.unibe.ch/id/eprint/146369 |
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