De Niz, Mariana; Kehrer, Jessica; Brancucci, Nicolas M B; Moalli, Federica; Reynaud, Emmanuel G; Stein, Jens V; Frischknecht, Friedrich (2020). 3D imaging of undissected optically cleared Anopheles stephensi mosquitoes and midguts infected with Plasmodium parasites. PLoS ONE, 15(9), e0238134. Public Library of Science 10.1371/journal.pone.0238134
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Malaria is a life-threatening disease, caused by Apicomplexan parasites of the Plasmodium genus. The Anopheles mosquito is necessary for the sexual replication of these parasites and for their transmission to vertebrate hosts, including humans. Imaging of the parasite within the insect vector has been attempted using multiple microscopy methods, most of which are hampered by the presence of the light scattering opaque cuticle of the mosquito. So far, most imaging of the Plasmodium mosquito stages depended on either sectioning or surgical dissection of important anatomical sites, such as the midgut and the salivary glands. Optical projection tomography (OPT) and light sheet fluorescence microscopy (LSFM) enable imaging fields of view in the centimeter scale whilst providing micrometer resolution. In this paper, we compare different optical clearing protocols and present reconstructions of the whole body of Plasmodium-infected, optically cleared Anopheles stephensi mosquitoes and their midguts. The 3D-reconstructions from OPT imaging show detailed features of the mosquito anatomy and enable overall localization of parasites in midguts. Additionally, LSFM imaging of mosquito midguts shows detailed distribution of oocysts in extracted midguts. This work was submitted as a pre-print to bioRxiv, available at https://www.biorxiv.org/content/10.1101/682054v2.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
04 Faculty of Medicine > Pre-clinic Human Medicine > Theodor Kocher Institute 08 Faculty of Science > Department of Biology > Institute of Cell Biology 08 Faculty of Science > Department of Biology > Institute of Cell Biology > Malaria |
Graduate School: |
Graduate School for Cellular and Biomedical Sciences (GCB) |
UniBE Contributor: |
De Niz Hidalgo, Mariana Isabel, Moalli, Federica |
Subjects: |
600 Technology > 610 Medicine & health 500 Science > 570 Life sciences; biology 500 Science |
ISSN: |
1932-6203 |
Publisher: |
Public Library of Science |
Funders: |
[42] Schweizerischer Nationalfonds |
Language: |
English |
Submitter: |
Volker Heussler |
Date Deposited: |
15 Oct 2020 17:01 |
Last Modified: |
05 Dec 2022 15:41 |
Publisher DOI: |
10.1371/journal.pone.0238134 |
PubMed ID: |
32936796 |
BORIS DOI: |
10.7892/boris.147065 |
URI: |
https://boris.unibe.ch/id/eprint/147065 |
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