Spheroid-Like Cultures for Expanding Angiopoietin Receptor-1 (aka. Tie2) Positive Cells from the Human Intervertebral Disc.

Zhang, Xingshuo; Guerrero, Julien; Croft, Andreas S.; Albers, Christoph E.; Häckel, Sonja; Gantenbein, Benjamin (2020). Spheroid-Like Cultures for Expanding Angiopoietin Receptor-1 (aka. Tie2) Positive Cells from the Human Intervertebral Disc. International journal of molecular sciences, 21(24) MDPI 10.3390/ijms21249423

[img]
Preview
Text
Zhang 2020 #2.pdf - Published Version
Available under License Creative Commons: Attribution (CC-BY).

Download (6MB) | Preview
[img]
Preview
Text (Supplementary files)
Zhang 2020 #3.pdf - Published Version
Available under License Creative Commons: Attribution (CC-BY).

Download (548kB) | Preview

Lower back pain is a leading cause of disability worldwide. The recovery of nucleus pulposus (NP) progenitor cells (NPPCs) from the intervertebral disc (IVD) holds high promise for future cell therapy. NPPCs are positive for the angiopoietin-1 receptor (Tie2) and possess stemness capacity. However, the limited Tie2+ NPC yield has been a challenge for their use in cell-based therapy for regenerative medicine. In this study, we attempted to expand NPPCs from the whole NP cell population by spheroid-formation assay. Flow cytometry was used to quantify the percentage of NPPCs with Tie2-antibody in human primary NP cells (NPCs). Cell proliferation was assessed using the population doublings level (PDL) measurement. Synthesis and presence of extracellular matrix (ECM) from NPC spheroids were confirmed by quantitative Polymerase Chain Reaction (qPCR), immunostaining, and microscopy. Compared with monolayer, the spheroid-formation assay enriched the percentage of Tie2+ in NPCs' population from ~10% to ~36%. Moreover, the spheroid-formation assay also inhibited the proliferation of the Tie2- NPCs with nearly no PDL. After one additional passage (P) using the spheroid-formation assay, NPC spheroids presented a Tie2+ percentage even further by ~10% in the NPC population. Our study concludes that the use of a spheroid culture system could be successfully applied to the culture and expansion of tissue-specific progenitors.

Item Type:

Journal Article (Original Article)

Division/Institute:

09 Interdisciplinary Units > Microscopy Imaging Center (MIC)
04 Faculty of Medicine > Department of Orthopaedic, Plastic and Hand Surgery (DOPH) > Clinic of Orthopaedic Surgery

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Zhang, Xingshuo; Guerrero, Julien Paul; Croft, Andreas Shaun; Albers, Christoph; Häckel, Sonja and Gantenbein, Benjamin

Subjects:

600 Technology > 610 Medicine & health
500 Science > 570 Life sciences; biology

ISSN:

1422-0067

Publisher:

MDPI

Language:

English

Submitter:

Benjamin Gantenbein

Date Deposited:

30 Dec 2020 15:58

Last Modified:

30 Dec 2020 15:58

Publisher DOI:

10.3390/ijms21249423

PubMed ID:

33322051

Uncontrolled Keywords:

angiopoietin-1 receptor (aka. Tie2) intervertebral disc (IVD) nucleus pulposus progenitor cells (NPPCs) spheroid-formation assay suspension culture

BORIS DOI:

10.7892/boris.149843

URI:

https://boris.unibe.ch/id/eprint/149843

Actions (login required)

Edit item Edit item
Provide Feedback