Neuropeptide Y modulates steroid production of human adrenal H295R cells through Y1 receptors

Kempná, Petra; Körner, Meike; Waser, Beatrice; Hofer, Gaby; Nuoffer, Jean-Marc; Reubi, J.C.; Flück, Christa E. (2010). Neuropeptide Y modulates steroid production of human adrenal H295R cells through Y1 receptors. Molecular and cellular endocrinology, 314(1), pp. 101-109. Shannon: Elsevier Ireland 10.1016/j.mce.2009.08.010

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Neuropeptide Y (NPY) is abundantly expressed in the nervous system and acts on target cells through NPY receptors. The human adrenal cortex and adrenal tumors express NPY receptor subtype Y1, but its function is unknown. We studied Y1-mediated signaling, steroidogenesis and cell proliferation in human adrenal NCI-H295R cells. Radioactive ligand binding studies showed that H295R cells express Y1 receptor specifically. NPY treatment of H295R cells stimulated the MEK/ERK1/2 pathway, confirming that H295R cells express functional Y1 receptors. Studies of the effect of NPY and related peptide PYY on adrenal steroidogenesis revealed a decrease in 11-deoxycortisol production. RIA measurements of cortisol from cell culture medium confirmed this finding. Co-treatment with the Y1 antagonist BIBP2336 reversed the inhibitory effect of NPY on cortisol production proving specificity of this effect. At mRNA level, NPY decreased HSD3B2 and CYP21A2 expression. However NPY revealed no effect on cell proliferation. Our data show that NPY can directly regulate human adrenal cortisol production.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute of Pathology
04 Faculty of Medicine > Department of Gynaecology, Paediatrics and Endocrinology (DFKE) > Clinic of Paediatric Medicine
04 Faculty of Medicine > Department of Haematology, Oncology, Infectious Diseases, Laboratory Medicine and Hospital Pharmacy (DOLS) > Institute of Clinical Chemistry

UniBE Contributor:

Kempna, Petra; Körner Jachertz, Meike; Waser, Beatrice; Hofer, Gaby; Nuoffer, Jean-Marc; Reubi-Kattenbusch, Jean-Claude and Flück, Christa

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

0303-7207

Publisher:

Elsevier Ireland

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:07

Last Modified:

14 Oct 2019 16:50

Publisher DOI:

10.1016/j.mce.2009.08.010

PubMed ID:

19699258

Web of Science ID:

000272101700012

BORIS DOI:

10.7892/boris.15

URI:

https://boris.unibe.ch/id/eprint/15 (FactScience: 165570)

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