Partially shielded enzymes capable of processing large protein substrates.

Briand, Manon L; Gebleux, Remy; Richina, Federica; Correro, M Rita; Grether, Yasmin; Dudal, Yves; Braga-Lagache, Sophie; Heller, Manfred; Beerli, Roger R; Grawunder, Ulf; Corvini, Philippe F-X; Shahgaldian, Patrick (2020). Partially shielded enzymes capable of processing large protein substrates. Chemical communications, 56(38), pp. 5170-5173. Royal Society of Chemistry 10.1039/d0cc01150a

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We report the first method of enzyme protection enabling the production of partially shielded enzymes capable of processing substrates as large as proteins. We show that partially shielded sortase retains its transpeptidase activity and can perform bioconjugation reactions on antibodies. Moreover, a partially shielded trypsin is shown to outperform its soluble counterpart in terms of proteolytic kinetics. Remarkably, partial enzyme shielding results in a drastic increase in temporal stability of the enzyme.

Item Type:

Journal Article (Review Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR)
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DCR Services > Core Facility Massenspektrometrie- und Proteomics-Labor
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Unit Childrens Hospital > Protein- und Zellbiologie

UniBE Contributor:

Braga, Sophie Marie-Pierre and Heller, Manfred

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1359-7345

Publisher:

Royal Society of Chemistry

Language:

English

Submitter:

Marla Rittiner

Date Deposited:

29 Dec 2020 10:37

Last Modified:

29 Dec 2020 10:37

Publisher DOI:

10.1039/d0cc01150a

PubMed ID:

32266896

BORIS DOI:

10.48350/150112

URI:

https://boris.unibe.ch/id/eprint/150112

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