Partially shielded enzymes capable of processing large protein substrates.

Briand, Manon L; Gebleux, Remy; Richina, Federica; Correro, M Rita; Grether, Yasmin; Dudal, Yves; Braga-Lagache, Sophie; Heller, Manfred; Beerli, Roger R; Grawunder, Ulf; Corvini, Philippe F-X; Shahgaldian, Patrick (2020). Partially shielded enzymes capable of processing large protein substrates. Chemical communications, 56(38), pp. 5170-5173. Royal Society of Chemistry 10.1039/d0cc01150a

Text d0cc01150a.pdf - Published Version Restricted to registered users only Available under License Publisher holds Copyright. Download (2MB)

We report the first method of enzyme protection enabling the production of partially shielded enzymes capable of processing substrates as large as proteins. We show that partially shielded sortase retains its transpeptidase activity and can perform bioconjugation reactions on antibodies. Moreover, a partially shielded trypsin is shown to outperform its soluble counterpart in terms of proteolytic kinetics. Remarkably, partial enzyme shielding results in a drastic increase in temporal stability of the enzyme.

Interest & Impact

Downloads

1 since deposited on 29 Dec 2020
0 in the past 12 months

Citations

5 Citations in Web of Science ®
6 Citations in Scopus

Search

in Google Scholar™

Services

Actions (login required)

Edit item

Actions (login required)

Edit item