tRNA 3' shortening by LCCR4 as a response to stress in Trypanosoma brucei

Cristodero, Marina; Brogli, Rebecca; Joss, Oliver; Schimanski, Bernd; Schneider, André; Polacek, Norbert (2021). tRNA 3' shortening by LCCR4 as a response to stress in Trypanosoma brucei. Nucleic acids research, 49(3), pp. 1647-1661. Oxford University Press 10.1093/nar/gkaa1261

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Sensing of environmental cues is crucial for cell survival. To adapt to changes in their surroundings cells need to tightly control the repertoire of genes expressed at any time. Regulation of translation is key, especially in organisms in which transcription is hardly controlled, like Trypanosoma brucei. In this study, we describe the shortening of the bulk of the cellular tRNAs during stress at the expense of the conserved 3' CCA-tail. This tRNA shortening is specific for nutritional stress and renders tRNAs unsuitable substrates for translation. We uncovered the nuclease LCCR4 (Tb927.4.2430), a homologue of the conserved deadenylase Ccr4, as being responsible for tRNA trimming. Once optimal growth conditions are restored tRNAs are rapidly repaired by the trypanosome tRNA nucleotidyltransferase thus rendering the recycled tRNAs amenable for translation. This mechanism represents a fast and efficient way to repress translation during stress, allowing quick reactivation with a low energy input.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Chemistry, Biochemistry and Pharmaceutical Sciences (DCBP)

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Cristodero, Marina; Brogli, Rebecca; Joss, Oliver; Schimanski, Bernd; Schneider, André and Polacek, Norbert

Subjects:

500 Science > 570 Life sciences; biology
500 Science > 540 Chemistry

ISSN:

0305-1048

Publisher:

Oxford University Press

Language:

English

Submitter:

Christina Schüpbach

Date Deposited:

29 Jan 2021 15:50

Last Modified:

23 Feb 2021 01:34

Publisher DOI:

10.1093/nar/gkaa1261

PubMed ID:

33406257

BORIS DOI:

10.48350/151090

URI:

https://boris.unibe.ch/id/eprint/151090

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