The influence of clinical severity and topical antimicrobial treatment on bacteriological culture and the microbiota of equine pastern dermatitis.

Sangiorgio, Daphne B.; Hilty, Markus; Kaiser-Thom, Sarah; Epper, Pascale G.; Ramseyer, Alessandra A.; Overesch, Gudrun; Gerber, Vinzenz M. (2021). The influence of clinical severity and topical antimicrobial treatment on bacteriological culture and the microbiota of equine pastern dermatitis. Veterinary dermatology, 32(2), 173-e41. Wiley 10.1111/vde.12912

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BACKGROUND

Equine pastern dermatitis (EPD) is a common dermatological problem in horses, yet its aetiology and pathogenesis are poorly understood.

OBJECTIVES

This study aimed to investigate the effects of lesion severity and topical antimicrobial treatment on bacterial flora of EPD-affected skin.

ANIMALS

Sixteen horses with EPD were investigated.

METHODS AND MATERIALS

An observational study was conducted by assigning a clinical severity score ranging from 0 (macroscopically nonlesional) to 21 (severe), and sampling the most and least severely affected limbs of 16 horses (32 limbs) for bacteriological culture and 16S rRNA sequencing. Topical antimicrobial treatment in the month before sampling was recorded. The limbs were allocated to a nonlesional or mildly affected group (Group A, score 0-3) and a moderate to severely affected group (Group B, score 4-21).

RESULTS

The most commonly cultured bacterial species was Staphylococcus aureus (one of 15 Group A versus nine of 17 Group B). Within Group B, S. aureus was found in three of six limbs treated with topical antimicrobials and in six of 11 untreated limbs. β-haemolytic streptococci (three of 32) and Trueperella pyogenes (two of 32) also were cultured exclusively in the untreated limbs of Group B. Staphylococci and streptococci were found more often by 16S rRNA sequencing than in culture. Limbs with higher lesion severity and topical antimicrobial treatment appeared to have a lower alpha diversity and different beta diversity compared to milder and untreated lesions.

CONCLUSIONS AND CLINICAL IMPORTANCE

Observed differences in microbiota of equine skin are likely to be linked to the presence and severity of EPD and topical antimicrobial treatment. Further research is needed to establish causal bacteria.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > Veterinary Public Health / Herd Health Management
04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases > Research
04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > Equine Clinic
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV)
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology

UniBE Contributor:

Sangiorgio, Daphne Belinda; Hilty, Markus; Kaiser-Thom, Sarah; Epper, Pascale Gabriela; Ramseyer, Alessandra; Overesch, Gudrun and Gerber, Vinzenz

Subjects:

600 Technology > 630 Agriculture
500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

1365-3164

Publisher:

Wiley

Language:

English

Submitter:

Ursula Therese Horst

Date Deposited:

18 Jan 2021 09:20

Last Modified:

19 Mar 2021 01:33

Publisher DOI:

10.1111/vde.12912

PubMed ID:

33417744

BORIS DOI:

10.48350/151359

URI:

https://boris.unibe.ch/id/eprint/151359

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