Monney, Thierry; Debache, Karim; Grandgirard, Denis; Leib, Stephen L.; Hemphill, Andrew (2012). Vaccination with the recombinant chimeric antigen recNcMIC3-1-R induces a non-protective Th2-type immune response in the pregnant mouse model for N. caninum infection. Vaccine, 30(46), pp. 6588-94. Amsterdam: Elsevier 10.1016/j.vaccine.2012.08.024
![[img]](https://boris.unibe.ch/style/images/fileicons/text.png) |
Text
__nas-ifik.unibe.ch_ifik_Forschung_Gr_Leib_SHARED GROUP FOLDER_own papers_Vaccination with the recombinant chimeric antigen recNcMIC3-1-R induces a.pdf - Published Version Restricted to registered users only Available under License Publisher holds Copyright. Download (353kB) |
The major route of transmission of Neospora caninum in cattle is transplacentally from an infected cow to its progeny. Therefore, a vaccine should be able to prevent both the horizontal transmission from contaminated food or water and the vertical transmission. We have previously shown that a chimeric vaccine composed of predicted immunogenic epitopes of NcMIC3, NcMIC1 and NcROP2 (recNcMIC3-1-R) significantly reduced the cerebral infection in BALB/c mice. In this study, mice were first vaccinated, then mated and pregnant mice were challenged with 2×10(6)N. caninum tachyzoites at day 7-9 of pregnancy. Partial protection was only observed in the mice vaccinated with a tachyzoite crude protein extract but no protection against vertical transmission or cerebral infection in the dams was observed in the group vaccinated with recNcMIC3-1-R. Serological and cytokine analysis showed an overall lower cytokine level in sera associated with a dominant IL-4 expression and high IgG1 titers. Thus, the Th2-type immune response observed in the pregnant mice was not protective against experimental neosporosis, in contrary to the mixed Th1-/Th2-type immune response observed in the non-pregnant mouse model. These results demonstrate that the immunomodulation that occurs during pregnancy was not favorable for the protection against N. caninum infection conferred by vaccination with recNcMIC3-1-R.
Actions (login required)
 |
Edit item |