In vitro immunoregulatory activity and anti-inflammatory effect of Echinococcus granulosus laminated layer.

Benazzouz, Sara; Amri, Manel; Wang, Junhua; Bouaziz, Samia; Ameur, Fahima; Djebbara, Sara; Achour, Karima; Gottstein, Bruno; Touil-Boukoffa, Chafia (2021). In vitro immunoregulatory activity and anti-inflammatory effect of Echinococcus granulosus laminated layer. Acta tropica, 218, p. 105886. Elsevier 10.1016/j.actatropica.2021.105886

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The Laminated layer of Echinococcus granulosus (LL) is the outer layer of the hydatic cyst. It plays a pivotal role in protecting the metacestode from host immunity. In our current study, we investigated the immunomodulatory effect of the LL on mouse spleen cells in presence of Lipopolysaccharide (LPS). Mouse spleen cells were cultured with or without LL in presence of LPS. After 24 h, the nitrites level representative of Nitric oxide (NO) production was measured in the culture supernatant by Griess-modified method. In addition, the mRNA expression levels of cytokines (IFN-γ, IL-1β, TGF-β, IL-10), Foxp3, and CTLA-4 were measured by quantitative Real-Time Polymerase chain reaction (qRT-PCR). Interestingly, our results showed a significant decrease (p< 0.01) in NO production and IFN-γ mRNA level (p< 0.001) from LPS- induced spleen cells in response to LL after 24h of culture. Moreover, LPS induced high level of IL-1β that was significantly (p<0.05) down regulated by LL. Importantly, mRNA levels of TGF-β (p< 0.01), Foxp3 and IL-10 (p< 0.05) were significantly upregulated by LL. In conclusion, our data indicated the in vitro immuno-regulatory and anti-inflammatory effects of the hydatic Laminated Layer on mouse spleen cells. These effects are related to an innate response implicating up-regulation of Foxp3, IL-10 and TGF-β expression and down-regulation of IFN-γ and IL-1β expression. LL could constitute a potential candidate for controlling inflammation during inflammatory disease.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases > Research
04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Parasitology

UniBE Contributor:

Wang, Junhua and Gottstein, Bruno

Subjects:

600 Technology > 630 Agriculture
500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

0001-706X

Publisher:

Elsevier

Language:

English

Submitter:

Achim Braun Parham

Date Deposited:

09 Aug 2021 16:38

Last Modified:

15 Aug 2021 03:10

Publisher DOI:

10.1016/j.actatropica.2021.105886

PubMed ID:

33713625

Uncontrolled Keywords:

Echinococcus granulosus Immune regulation Inflammation LPS Laminated layer Spleen cells

BORIS DOI:

10.48350/157898

URI:

https://boris.unibe.ch/id/eprint/157898

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