ATG5 and ATG7 Expression Levels Are Reduced in Cutaneous Melanoma and Regulated by NRF1.

Frangez, Ziva; Gérard, Deborah; He, Zhaoyue; Gavriil, Marios; Fernández-Marrero, Yuniel; Jafari, S. Morteza Seyed; Hunger, Robert E.; Lucarelli, Philippe; Yousefi, Shida; Sauter, Thomas; Sinkkonen, Lasse; Simon, Hans-Uwe (2021). ATG5 and ATG7 Expression Levels Are Reduced in Cutaneous Melanoma and Regulated by NRF1. Frontiers in oncology, 11, p. 721624. Frontiers Research Foundation 10.3389/fonc.2021.721624

[img]
Preview
Text
Simon_ATG5_and_ATG7_Expression_Levels.pdf - Published Version
Available under License Creative Commons: Attribution (CC-BY).

Download (6MB) | Preview

Autophagy is a highly conserved cellular process in which intracellular proteins and organelles are sequestered and degraded after the fusion of double-membrane vesicles known as autophagosomes with lysosomes. The process of autophagy is dependent on autophagy-related (ATG) proteins. The role of autophagy in cancer is very complex and still elusive. We investigated the expression of ATG proteins in benign nevi, primary and metastatic melanoma tissues using customized tissue microarrays (TMA). Results from immunohistochemistry show that the expression of ATG5 and ATG7 is significantly reduced in melanoma tissues compared to benign nevi. This reduction correlated with changes in the expression of autophagic activity markers, suggesting decreased basal levels of autophagy in primary and metastatic melanomas. Furthermore, the analysis of survival data of melanoma patients revealed an association between reduced ATG5 and ATG7 levels with an unfavourable clinical outcome. Currently, the mechanisms regulating ATG expression levels in human melanoma remains unknown. Using bioinformatic predictions of transcription factor (TF) binding motifs in accessible chromatin of primary melanocytes, we identified new TFs involved in the regulation of core ATGs. We then show that nuclear respiratory factor 1 (NRF1) stimulates the production of mRNA and protein as well as the promoter activity of ATG5 and ATG7. Moreover, NRF1 deficiency increased in vitro migration of melanoma cells. Our results support the concept that reduced autophagic activity contributes to melanoma development and progression, and identifies NRF1 as a novel TF involved in the regulation of both ATG5 and ATG7 genes.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Dermatology, Urology, Rheumatology, Nephrology, Osteoporosis (DURN) > Clinic of Dermatology
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Pharmacology

UniBE Contributor:

Frangez, Ziva; He, Zhaoyue; Fernández Marrero, Yuniel; Jafari, Morteza; Hunger, Robert; Yousefi, Shida and Simon, Hans-Uwe

Subjects:

600 Technology > 610 Medicine & health

ISSN:

2234-943X

Publisher:

Frontiers Research Foundation

Language:

English

Submitter:

Celine Joray

Date Deposited:

07 Sep 2021 14:51

Last Modified:

12 Sep 2021 03:18

Publisher DOI:

10.3389/fonc.2021.721624

PubMed ID:

34458153

Uncontrolled Keywords:

ATG5 ATG7 NRF1 autophagy melanoma transcription factor

BORIS DOI:

10.48350/159215

URI:

https://boris.unibe.ch/id/eprint/159215

Actions (login required)

Edit item Edit item
Provide Feedback