Validation of Immunohistochemistry for Canine Proteins Involved in Thyroid Iodine Uptake and Their Expression in Canine Follicular Cell Thyroid Carcinomas (FTCs) and FTC-Derived Organoids.

Jankovic, Jana; Dettwiler, Martina; González Fernández, Martin; Tièche, Eve; Hahn, Kerstin; April-Monn, Simon; Dettmer, Matthias S; Kessler, Martin; Rottenberg, Sven; Campos, Miguel (2021). Validation of Immunohistochemistry for Canine Proteins Involved in Thyroid Iodine Uptake and Their Expression in Canine Follicular Cell Thyroid Carcinomas (FTCs) and FTC-Derived Organoids. Veterinary pathology, 58(6), pp. 1172-1180. Sage 10.1177/03009858211018813

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Thyrotropin receptor (TSHR), sodium iodide symporter (NIS), pendrin, and thyroid peroxidase (TPO) are essential for the uptake of iodine by follicular thyroid cells. The aim of this study was to establish immunohistochemistry (IHC) protocols for TSHR, NIS, pendrin, and TPO in canine tissues and characterize their expression in organoids derived from canine follicular cell thyroid carcinoma (FTC) and in the respective primary tumors. This constitutes a fundamental step to establish organoids as a model to study the uptake of iodine in canine FTC. Commercially available antibodies directed against human proteins were selected. Antibody specificity was confirmed by western blot using lysates of the HTori-3 human thyroid cell line and healthy canine thyroid gland. IHC was validated using HTori-3 cells and a set of canine normal tissues including healthy thyroid gland. The expression of TSHR, NIS, pendrin, and TPO was evaluated in 3 organoid lines derived from FTC and respective primary tumors. All 4 antibodies produced specific bands by western blot and cytoplasmic labeling in follicular cells by IHC in both human HTori-3 cells and canine thyroid gland. NIS also showed basolateral membrane immunolabeling in follicular cells. All 4 proteins were highly expressed in organoids derived from FTC. The expression was similar or higher compared to the primary tumors. The results of this study characterize organoids derived from canine FTC as a suitable in vitro model to investigate iodine uptake, opening new research possibilities in the field of canine thyroid cancer therapy.

Item Type:	Journal Article (Original Article)
Division/Institute:	04 Faculty of Medicine > Service Sector > Institute of Pathology 05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) 05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > Small Animal Clinic > Small Animal Clinic, Internal Medicine 05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > Small Animal Clinic 05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Animal Pathology 05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) 05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > DKV - Anaesthesiology
UniBE Contributor:	Jankovic, Jana, Dettwiler, Martina Andrea, Gonzalez Fernandez, Martin, Dettmer, Matthias, Rottenberg, Sven, Campos, Miguel
Subjects:	500 Science > 570 Life sciences; biology 600 Technology > 610 Medicine & health 600 Technology > 630 Agriculture
ISSN:	1544-2217
Publisher:	Sage
Language:	English
Submitter:	Simone Schuller
Date Deposited:	13 Sep 2021 13:18
Last Modified:	18 Dec 2023 12:54
Publisher DOI:	10.1177/03009858211018813
PubMed ID:	34056980
Uncontrolled Keywords:	dogs endocrine gland neoplasms immunohistochemistry iodine metabolism organoids thyroid carcinoma thyroid-stimulating hormone receptor western blot
URI:	https://boris.unibe.ch/id/eprint/159271