Urinary miRNA profiles discriminate between obstruction-induced bladder dysfunction and healthy controls.

von Siebenthal, Michelle; Besic, Mustafa; Hashemi Gheinani, Ali; Akshay, Akshay; Lizun-Platoni, Salomé; Kunz, Nadine; Burkhard, Fiona C.; Monastyrskaya, Katia (2021). Urinary miRNA profiles discriminate between obstruction-induced bladder dysfunction and healthy controls. Scientific reports, 11(1), p. 10204. Springer Nature 10.1038/s41598-021-89535-3

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Urgency, frequency and incomplete emptying are the troublesome symptoms often shared between benign prostatic obstruction-induced (BLUTD) and neurogenic (NLUTD) lower urinary tract dysfunction. Previously, using bladder biopsies, we suggested a panel of miRNA biomarkers for different functional phenotypes of the bladder. Urine is a good source of circulating miRNAs, but sex- and age-matched controls are important for urinary metabolite comparison. In two groups of healthy subjects (average age 32 and 57 years old, respectively) the total protein and RNA content was very similar between age groups, but the number of secreted extracellular vesicles (uEVs) and expression of several miRNAs were higher in the young healthy male volunteers. Timing of urine collection was not important for these parameters. We also evaluated the suitability of urinary miRNAs for non-invasive diagnosis of bladder outlet obstruction (BOO). A three urinary miRNA signature (miR-10a-5p, miR-301b-3p and miR-363-3p) could discriminate between controls and patients with LUTD (BLUTD and NLUTD). This panel of representative miRNAs can be further explored to develop a non-invasive diagnostic test for BOO. The age-related discrepancy in the urinary miRNA content observed in this study points to the importance of selecting appropriate, age-matched controls.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Urologie
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Urologie

04 Faculty of Medicine > Department of Dermatology, Urology, Rheumatology, Nephrology, Osteoporosis (DURN) > Clinic of Urology
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR)

UniBE Contributor:

von Siebenthal, Michelle Andrea; Besic, Mustafa; Akshay, Akshay; Burkhard, Fiona Christine and Monastyrskaya-Stäuber, Katia

Subjects:

600 Technology > 610 Medicine & health

ISSN:

2045-2322

Publisher:

Springer Nature

Language:

English

Submitter:

Jeannine Wiemann

Date Deposited:

13 Oct 2021 10:22

Last Modified:

17 Oct 2021 03:09

Publisher DOI:

10.1038/s41598-021-89535-3

PubMed ID:

33986358

BORIS DOI:

10.48350/159669

URI:

https://boris.unibe.ch/id/eprint/159669

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