Novel biomarkers for the early prediction of pediatric cystic echinococcosis post-surgical outcomes.

Ben Salah, Eya; Barrera, Coralie; Sakly, Wahiba; Mosbahi, Sana; Balliau, Thierry; Franche, Nathalie; Gottstein, Bruno; Ben Youssef, Sabrine; Mekki, Mongi; Babba, Hamouda; Millon, Laurence (2022). Novel biomarkers for the early prediction of pediatric cystic echinococcosis post-surgical outcomes. Journal of infection, 84(1), pp. 87-93. Elsevier 10.1016/j.jinf.2021.09.023

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OBJECTIVE

This study aims to search for reliable serological biomarkers allowing the early prediction of cystic echinococcosis (CE) post-operative outcomes.

METHODS

We applied immunoprecipitation (IP) of Echinococcus granulosus protoscolex antigens with pediatric CE patients' plasma collected at 1-month and 1-year post-surgery, followed by Liquid Chromatography with tandem mass spectrometry (LC-MS/MS). We compared IP proteomic content from relapsed patients within the first-year post-surgery (RCE) to cases with no relapses until 3 post-operative years (NRCE). Selected proteins were recombinantly synthesized and assessed for their prognostic performance by Enzyme-linked immunosorbent assay (ELISA).

RESULTS

A total of 305 immunoreactive parasitic proteins were identified, 59 of which were significantly more abundant in RCE than NRCE for both time-points. Four proteins showed the most promising characteristics for predicting CE outcomes: cytoplasmic malate dehydrogenase (Eg-cMDH), citrate synthase (Eg-CS), annexin A6 and severin. ELISA-IgG against the four markers were significantly lower at 1-year post-surgery than 1-month in NRCE, in contrast to RCE that displayed either stable or higher levels. The Eg-cMDH and Eg-CS showed the best prognostic performance, with respective probabilities of being "relapse-free" of 83% and 81%, if a decrease of IgG levels occurred between 1-month and 1-year post-surgery.

CONCLUSION

The Eg-cMDH and Eg-CS are promising biomarkers to predict early CE post-surgical outcomes.

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