Genotyping Echinococcus multilocularis in Human Alveolar Echinococcosis Patients: An EmsB Microsatellite Analysis.

Knapp, Jenny; Gottstein, Bruno; Bretagne, Stéphane; Bart, Jean-Mathieu; Umhang, Gérald; Richou, Carine; Bresson-Hadni, Solange; Millon, Laurence (2020). Genotyping Echinococcus multilocularis in Human Alveolar Echinococcosis Patients: An EmsB Microsatellite Analysis. Pathogens, 9(4) MDPI AG 10.3390/pathogens9040282

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For clinical epidemiology specialists, connecting the genetic diversity of Echinococcus multilocularis to sources of infection or particular sites has become somewhat of a holy grail. It is very difficult to trace the infection history of alveolar echinococcosis (AE) patients as there may be an incubation period of five to 15 years before reliable diagnosis. Moreover, the variability of parasitic manifestations in human patients raises the possibility of genetically different isolates of E. multilocularis having different levels of pathogenicity. Thus, the exposure of human patients to different strains or genotypes circulating in geographically different environments may lead to different disease outcomes. Molecular tools, such as the microsatellite marker EmsB, were required to investigate these aspects. This genetic marker was previously tested on a collection of 1211 European field samples predominantly of animal origin, referenced on a publicly available database. In this study, we investigated a panel of 66 metacestode samples (between 1981 and 2019) recovered surgically from 63 patients diagnosed with alveolar echinococcosis originating from four European countries (France, Switzerland, Germany, Belgium). In this study, we identified nine EmsB profiles, five of which were found in patients located in the same areas of France and Switzerland. One profile was detected on both sides of the French-Swiss border, whereas most patients from non-endemic regions clustered together in another profile. EmsB profiles appeared to remain stable over time because similar profiles were detected in patients who underwent surgery recently and patients who underwent surgery some time ago. This study sheds light on possible pathways of contamination in humans, including proximity contamination in some cases, and the dominant contamination profiles in Europe, particularly for extrahepatic lesions.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases > Research
04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases > Parasitology
04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases

UniBE Contributor:

Gottstein, Bruno

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

2076-0817

Publisher:

MDPI AG

Language:

English

Submitter:

Bruno Gottstein

Date Deposited:

26 Nov 2021 13:34

Last Modified:

05 Dec 2022 15:55

Publisher DOI:

10.3390/pathogens9040282

PubMed ID:

32295095

Uncontrolled Keywords:

Alveolar echinococcosis Echinococcus multilocularis European endemic area contamination event genotyping microsatellite EmsB

BORIS DOI:

10.48350/161596

URI:

https://boris.unibe.ch/id/eprint/161596

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