Rhesus Macaque CODEX Multiplexed Immunohistochemistry Panel for Studying Immune Responses During Ebola Infection.

Jiang, Sizun; Mukherjee, Nilanjan; Bennett, Richard S; Chen, Han; Logue, James; Dighero-Kemp, Bonnie; Kurtz, Jonathan R; Adams, Ricky; Phillips, Darci; Schürch, Christian M; Goltsev, Yury; Hickey, John W; McCaffrey, Erin F; Delmastro, Alea; Chu, Pauline; Reader, J Rachel; Keesler, Rebekah I; Galván, José A.; Zlobec, Inti; Van Rompay, Koen K A; ... (2021). Rhesus Macaque CODEX Multiplexed Immunohistochemistry Panel for Studying Immune Responses During Ebola Infection. Frontiers in immunology, 12, p. 729845. Frontiers Research Foundation 10.3389/fimmu.2021.729845

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Non-human primate (NHP) animal models are an integral part of the drug research and development process. For some biothreat pathogens, animal model challenge studies may offer the only possibility to evaluate medical countermeasure efficacy. A thorough understanding of host immune responses in such NHP models is therefore vital. However, applying antibody-based immune characterization techniques to NHP models requires extensive reagent development for species compatibility. In the case of studies involving high consequence pathogens, further optimization for use of inactivated samples may be required. Here, we describe the first optimized CO-Detection by indEXing (CODEX) multiplexed tissue imaging antibody panel for deep profiling of spatially resolved single-cell immune responses in rhesus macaques. This 21-marker panel is composed of a set of 18 antibodies that stratify major immune cell types along with a set three Ebola virus (EBOV)-specific antibodies. We validated these two sets of markers using immunohistochemistry and CODEX in fully inactivated Formalin-Fixed Paraffin-Embedded (FFPE) tissues from mock and EBOV challenged macaques respectively and provide an efficient framework for orthogonal validation of multiple antibody clones using CODEX multiplexed tissue imaging. We also provide the antibody clones and oligonucleotide tag sequences as a valuable resource for other researchers to recreate this reagent set for future studies of tissue immune responses to EBOV infection and other diseases.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute of Pathology
04 Faculty of Medicine > Service Sector > Institute of Pathology > Translational Research Unit

UniBE Contributor:

Galván Hernández, José Alberto and Zlobec, Inti

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

1664-3224

Publisher:

Frontiers Research Foundation

Language:

English

Submitter:

José Alberto Galván Hernández

Date Deposited:

29 Dec 2021 14:46

Last Modified:

02 Jan 2022 01:54

Publisher DOI:

10.3389/fimmu.2021.729845

PubMed ID:

34938283

Uncontrolled Keywords:

EBOV (Ebola virus) NHP (non-human primate) Spatial biology codex multiplexed immunofluorescencence and immunohistochemistry rhesus macaque (Macaca mulatta tcheliensis)

BORIS DOI:

10.48350/163458

URI:

https://boris.unibe.ch/id/eprint/163458

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