RNA degradation differentially affects quantitative mRNA measurements of endogenous reference genes in human placenta

Huang, Xiao; Baumann, M; Nikitina, L; Wenger, F; Surbek, D; Körner, M; Albrecht, Christiane (2013). RNA degradation differentially affects quantitative mRNA measurements of endogenous reference genes in human placenta. Placenta, 34(7), pp. 544-7. Amsterdam: Elsevier 10.1016/j.placenta.2013.03.011

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It has been highlighted that RNA quality and appropriate reference gene selection is crucial for the interpretation of RT-qPCR results in human placental samples. In this context we investigated the effect of RNA degradation on the mRNA abundance of seven frequently used reference genes in 119 human placental samples. Combining RNA integrity measurements, RT-qPCR analysis and mathematical modeling we found major differences regarding the effect of RNA degradation on the measured expression levels between the different reference genes. Furthermore, we demonstrated that a modified RNA extraction method significantly improved RNA quality and consequently increased transcript levels of all reference genes.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Biochemistry and Molecular Medicine
04 Faculty of Medicine > Department of Gynaecology, Paediatrics and Endocrinology (DFKE) > Clinic of Gynaecology

UniBE Contributor:

Huang, Xiao; Baumann, Marc; Surbek, Daniel and Albrecht, Christiane

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

0143-4004

Publisher:

Elsevier

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:40

Last Modified:

03 Mar 2014 14:42

Publisher DOI:

10.1016/j.placenta.2013.03.011

PubMed ID:

23623484

Web of Science ID:

000320827100005

URI:

https://boris.unibe.ch/id/eprint/16489 (FactScience: 224139)

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