Huang, Xiao; Baumann, M; Nikitina, L; Wenger, F; Surbek, D; Körner, M; Albrecht, Christiane (2013). RNA degradation differentially affects quantitative mRNA measurements of endogenous reference genes in human placenta. Placenta, 34(7), pp. 544-7. Amsterdam: Elsevier 10.1016/j.placenta.2013.03.011
Full text not available from this repository. (Request a copy)It has been highlighted that RNA quality and appropriate reference gene selection is crucial for the interpretation of RT-qPCR results in human placental samples. In this context we investigated the effect of RNA degradation on the mRNA abundance of seven frequently used reference genes in 119 human placental samples. Combining RNA integrity measurements, RT-qPCR analysis and mathematical modeling we found major differences regarding the effect of RNA degradation on the measured expression levels between the different reference genes. Furthermore, we demonstrated that a modified RNA extraction method significantly improved RNA quality and consequently increased transcript levels of all reference genes.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Biochemistry and Molecular Medicine 04 Faculty of Medicine > Department of Gynaecology, Paediatrics and Endocrinology (DFKE) > Clinic of Gynaecology |
UniBE Contributor: |
Huang, Xiao, Baumann, Marc, Surbek, Daniel, Albrecht, Christiane |
Subjects: |
500 Science > 570 Life sciences; biology 600 Technology > 610 Medicine & health |
ISSN: |
0143-4004 |
Publisher: |
Elsevier |
Language: |
English |
Submitter: |
Factscience Import |
Date Deposited: |
04 Oct 2013 14:40 |
Last Modified: |
05 Dec 2022 14:12 |
Publisher DOI: |
10.1016/j.placenta.2013.03.011 |
PubMed ID: |
23623484 |
Web of Science ID: |
000320827100005 |
URI: |
https://boris.unibe.ch/id/eprint/16489 (FactScience: 224139) |
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