Paes Dias, Mariana; Rottenberg, Sven; Jonkers, Jos (2022). Functional genetic dropout screens and in vivo validation of candidate therapeutic targets using mouse mammary tumoroids. STAR protocols, 3(1), p. 101132. Elsevier 10.1016/j.xpro.2022.101132
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We have previously developed 3D tumoroids derived from genetically engineered mouse models (GEMMs) of BRCA1/2-deficient breast cancer. Here, we describe how to genetically modify tumoroids, use them for functional genetic dropout screens, and carry out orthotopic transplantation of modified tumoroids. The purpose of this protocol is to screen for therapeutic targets and allow rapid and straightforward in vivo validation of the candidate targets.
Item Type: |
Journal Article (Further Contribution) |
|---|---|
Division/Institute: |
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Animal Pathology |
Graduate School: |
Graduate School for Cellular and Biomedical Sciences (GCB) |
UniBE Contributor: |
Rottenberg, Sven |
Subjects: |
500 Science > 570 Life sciences; biology |
ISSN: |
2666-1667 |
Publisher: |
Elsevier |
Funders: |
[4] Swiss National Science Foundation ; [18] European Research Council ; [193] Swiss Cancer League = Krebsliga Schweiz |
Projects: |
Projects 310030 not found. Projects 883877 not found. Projects 4282 not found. |
Language: |
English |
Submitter: |
Sven Rottenberg |
Date Deposited: |
23 Feb 2022 15:05 |
Last Modified: |
05 Dec 2022 16:09 |
Publisher DOI: |
10.1016/j.xpro.2022.101132 |
PubMed ID: |
35146447 |
BORIS DOI: |
10.48350/165749 |
URI: |
https://boris.unibe.ch/id/eprint/165749 |
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