Biomolecules capturing live bacteria from clinical samples.

Sorgenfrei, Michèle; Hürlimann, Lea M; Remy, Mélissa M; Keller, Peter Michael; Seeger, Markus A (2022). Biomolecules capturing live bacteria from clinical samples. Trends in biochemical sciences, 47(8), pp. 673-688. Elsevier Current Trends 10.1016/j.tibs.2022.03.018

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Rapid phenotypic antimicrobial susceptibility testing (AST) requires the enrichment of live bacteria from patient samples, which is particularly challenging in the context of life-threatening bloodstream infections (BSIs) due to low bacterial titers. Over two decades, an extensive array of pathogen-specific biomolecules has been identified to capture live bacteria. The prevailing biomolecules are immune proteins of the complement system, antibodies, aptamers, phage proteins, and antimicrobial peptides. These biomolecules differ by their binder generation technologies and exhibit highly variable specificities, ranging from bacterial strains to most pathogenic bacteria. Here, we summarize how these diverse biomolecules were identified, list examples of successfully reported capture assays, and provide an outlook on the use of nanobodies raised against conserved surface-accessible proteins as promising biomolecules for pathogen capture.

Item Type:

Journal Article (Review Article)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases > Research
04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases

UniBE Contributor:

Remy, Mélissa, Keller, Peter Michael

Subjects:

600 Technology > 610 Medicine & health

ISSN:

0968-0004

Publisher:

Elsevier Current Trends

Language:

English

Submitter:

Pubmed Import

Date Deposited:

02 May 2022 09:12

Last Modified:

05 Dec 2022 16:19

Publisher DOI:

10.1016/j.tibs.2022.03.018

PubMed ID:

35487808

Uncontrolled Keywords:

antibodies antimicrobial resistance (AMR) aptamers immune proteins phage proteins rapid diagnostics

BORIS DOI:

10.48350/169653

URI:

https://boris.unibe.ch/id/eprint/169653

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