Spatial metabolomics for evaluating response to neoadjuvant therapy in non-small cell lung cancer patients.

Shen, Jian; Sun, Na; Zens, Philipp; Kunzke, Thomas; Buck, Achim; Prade, Verena M; Wang, Jun; Wang, Qian; Hu, Ronggui; Feuchtinger, Annette; Berezowska, Sabina Anna; Walch, Axel (2022). Spatial metabolomics for evaluating response to neoadjuvant therapy in non-small cell lung cancer patients. Cancer communications, 42(6), pp. 517-535. Wiley 10.1002/cac2.12310

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BACKGROUND

The response to neoadjuvant chemotherapy (NAC) differs substantially among individual patients with non-small cell lung cancer (NSCLC). Major pathological response (MPR) is a histomorphological read-out used to assess treatment response and prognosis in patients NSCLC after NAC. Although spatial metabolomics is a promising tool for evaluating metabolic phenotypes, it has not yet been utilized to assess therapy responses in patients with NSCLC. We evaluated the potential application of spatial metabolomics in cancer tissues to assess the response to NAC, using a metabolic classifier that utilizes mass spectrometry imaging combined with machine learning.

METHODS

Resected NSCLC tissue specimens obtained after NAC (n = 88) were subjected to high-resolution mass spectrometry, and these data were used to develop an approach for assessing the response to NAC in patients with NSCLC. The specificities of the generated tumor cell and stroma classifiers were validated by applying this approach to a cohort of biologically matched chemotherapy-naïve patients with NSCLC (n = 85).

RESULTS

The developed tumor cell metabolic classifier stratified patients into different prognostic groups with 81.6% accuracy, whereas the stroma metabolic classifier displayed 78.4% accuracy. By contrast, the accuracies of MPR and TNM staging for stratification were 62.5% and 54.1%, respectively. The combination of metabolic and MPR classifiers showed slightly lower accuracy than either individual metabolic classifier. In multivariate analysis, metabolic classifiers were the only independent prognostic factors identified (tumor: P = 0.001, hazards ratio [HR] = 3.823, 95% confidence interval [CI] = 1.716-8.514; stroma: P = 0.049, HR = 2.180, 95% CI = 1.004-4.737), whereas MPR (P = 0.804; HR = 0.913; 95% CI = 0.445-1.874) and TNM staging (P = 0.078; HR = 1.223; 95% CI = 0.977-1.550) were not independent prognostic factors. Using Kaplan-Meier survival analyses, both tumor and stroma metabolic classifiers were able to further stratify patients as NAC responders (P < 0.001) and non-responders (P < 0.001).

CONCLUSIONS

Our findings indicate that the metabolic constitutions of both tumor cells and the stroma are valuable additions to the classical histomorphology-based assessment of tumor response.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute of Pathology

Graduate School:

Graduate School for Health Sciences (GHS)

UniBE Contributor:

Zens, Philipp Immanuel and Berezowska, Sabina Anna

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

2523-3548

Publisher:

Wiley

Language:

English

Submitter:

Pubmed Import

Date Deposited:

23 May 2022 14:12

Last Modified:

16 Jun 2022 00:15

Publisher DOI:

10.1002/cac2.12310

PubMed ID:

35593195

Uncontrolled Keywords:

Non-small cell lung cancer cancer metabolism machine learning mass spectrometry imaging metabolic classifier prognosis spatial metabolomics treatment response

BORIS DOI:

10.48350/170150

URI:

https://boris.unibe.ch/id/eprint/170150

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