Analysis of Oligonucleotide Biodistribution and Metabolization in Experimental Animals.

Halloy, François; Brönnimann, Paulina; Hall, Jonathan; Schümperli, Daniel (2022). Analysis of Oligonucleotide Biodistribution and Metabolization in Experimental Animals. Methods in molecular biology, 2537, pp. 335-350. Springer 10.1007/978-1-0716-2521-7_19

Full text not available from this repository.

We describe methods to follow the fate of oligonucleotides after their injection into experimental animals. The quantitation in various tissues, blood or bone marrow cells is possible by chemical ligation PCR. This method works independently of chemical modifications of the oligonucleotide and/or its conjugations to lipid or peptide moieties. Moreover, metabolization intermediates can be detected by mass spectrometry. Together with a readout assay for the biochemical or physiological effects, which will differ, depending on the particular purpose of the oligonucleotide, these methods allow for a comprehensive understanding of oligonucleotide behavior in a living organism.

Item Type:

Journal Article (Further Contribution)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute of Pathology
04 Faculty of Medicine > Service Sector > Institute of Pathology > Translational Research Unit

UniBE Contributor:

Brönnimann, Paulina

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1940-6029

Publisher:

Springer

Language:

English

Submitter:

Pubmed Import

Date Deposited:

28 Jul 2022 15:44

Last Modified:

05 Dec 2022 16:22

Publisher DOI:

10.1007/978-1-0716-2521-7_19

PubMed ID:

35895273

Uncontrolled Keywords:

Chemical ligation qPCR Mass spectrometry Oligonucleotide biodistribution Oligonucleotide conjugates Oligonucleotide therapeutics

URI:

https://boris.unibe.ch/id/eprint/171587

Actions (login required)

Edit item Edit item
Provide Feedback