Halloy, François; Brönnimann, Paulina; Hall, Jonathan; Schümperli, Daniel (2022). Analysis of Oligonucleotide Biodistribution and Metabolization in Experimental Animals. Methods in molecular biology, 2537, pp. 335-350. Springer 10.1007/978-1-0716-2521-7_19
Full text not available from this repository. (Request a copy)We describe methods to follow the fate of oligonucleotides after their injection into experimental animals. The quantitation in various tissues, blood or bone marrow cells is possible by chemical ligation PCR. This method works independently of chemical modifications of the oligonucleotide and/or its conjugations to lipid or peptide moieties. Moreover, metabolization intermediates can be detected by mass spectrometry. Together with a readout assay for the biochemical or physiological effects, which will differ, depending on the particular purpose of the oligonucleotide, these methods allow for a comprehensive understanding of oligonucleotide behavior in a living organism.
Item Type: |
Journal Article (Further Contribution) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > Service Sector > Institute of Pathology 04 Faculty of Medicine > Service Sector > Institute of Pathology > Translational Research Unit |
UniBE Contributor: |
Brönnimann, Paulina |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
1940-6029 |
Publisher: |
Springer |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
28 Jul 2022 15:44 |
Last Modified: |
05 Dec 2022 16:22 |
Publisher DOI: |
10.1007/978-1-0716-2521-7_19 |
PubMed ID: |
35895273 |
Uncontrolled Keywords: |
Chemical ligation qPCR Mass spectrometry Oligonucleotide biodistribution Oligonucleotide conjugates Oligonucleotide therapeutics |
URI: |
https://boris.unibe.ch/id/eprint/171587 |
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