Immunohistochemical Detection of the Chaperone-Mediated Autophagy Markers LAMP2A and HSPA8 in Formalin-Fixed and Paraffin-Embedded Tissues.

Losmanová, Tereza; Tschan, Mario P; Galván, José A; Berezowska, Sabina (2023). Immunohistochemical Detection of the Chaperone-Mediated Autophagy Markers LAMP2A and HSPA8 in Formalin-Fixed and Paraffin-Embedded Tissues. Methods in molecular biology, 2566, pp. 141-147. Springer 10.1007/978-1-0716-2675-7_11

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Autophagy is crucial for maintaining cellular homeostasis and its deregulation is involved in disease development, including cancer. The key players of chaperone-mediated autophagy (CMA), a particular selective subtype of autophagy, are HSPA8 and LAMP2A. Both proteins can be immunohistochemically detected in formalin-fixed paraffin-embedded (FFPE) tissue. LAMP2A is frequently overexpressed in a variety of cancers where it likely supports cancer cell survival and resistance to anti-cancer therapies in a context-dependent manner. Here we present the immunohistochemical staining protocol of antibodies against LAMP2A and HSPA8, using an automated staining system, suitable for routine diagnostics. Additionally, we also suggest a staining evaluation method.

Item Type:

Journal Article (Further Contribution)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute of Pathology

UniBE Contributor:

Losmanová, Tereza; Tschan, Mario; Galván Hernández, José Alberto and Berezowska, Sabina Anna

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

1940-6029

Publisher:

Springer

Language:

English

Submitter:

Pubmed Import

Date Deposited:

26 Sep 2022 09:23

Last Modified:

05 Dec 2022 16:25

Publisher DOI:

10.1007/978-1-0716-2675-7_11

PubMed ID:

36152248

Uncontrolled Keywords:

CMA Chaperone-mediated autophagy HSPA8 Immunohistochemistry LAMP2A

BORIS DOI:

10.48350/173256

URI:

https://boris.unibe.ch/id/eprint/173256

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