Immunohistochemical Detection of the Autophagy Markers LC3 and p62/SQSTM1 in Formalin-Fixed and Paraffin-Embedded Tissue.

Berezowska, Sabina; Galván, José A (2023). Immunohistochemical Detection of the Autophagy Markers LC3 and p62/SQSTM1 in Formalin-Fixed and Paraffin-Embedded Tissue. Methods in molecular biology, 2566, pp. 133-139. Springer 10.1007/978-1-0716-2675-7_10

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Autophagy is a highly conserved cellular mechanism of "self-digestion," ensuring cellular homeostasis and playing a role in many diseases including cancer. As a stress response mechanism, it may also be involved in cellular response to therapy. LC3 and Sequestosome 1 (p62/SQSTM1) are among the most widely used markers to monitor autophagy and can be visualized in formalin-fixed and paraffin-embedded tissue by immunohistochemistry. Here we describe a validated staining protocol using an automated staining system available in many routine pathology laboratories, enabling high-throughput staining under standardized conditions.

Item Type:

Journal Article (Further Contribution)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute of Pathology
04 Faculty of Medicine > Service Sector > Institute of Pathology > Clinical Pathology

UniBE Contributor:

Berezowska, Sabina Anna and Galván Hernández, José Alberto

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

1940-6029

Publisher:

Springer

Language:

English

Submitter:

Pubmed Import

Date Deposited:

26 Sep 2022 08:38

Last Modified:

05 Dec 2022 16:25

Publisher DOI:

10.1007/978-1-0716-2675-7_10

PubMed ID:

36152247

Uncontrolled Keywords:

Autophagy Immunohistochemistry LC3 Sequestosome 1 p62/SQSTM1

BORIS DOI:

10.48350/173257

URI:

https://boris.unibe.ch/id/eprint/173257

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