Berezowska, Sabina; Galván, José A (2023). Immunohistochemical Detection of the Autophagy Markers LC3 and p62/SQSTM1 in Formalin-Fixed and Paraffin-Embedded Tissue. Methods in molecular biology, 2566, pp. 133-139. Springer 10.1007/978-1-0716-2675-7_10
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Autophagy is a highly conserved cellular mechanism of "self-digestion," ensuring cellular homeostasis and playing a role in many diseases including cancer. As a stress response mechanism, it may also be involved in cellular response to therapy. LC3 and Sequestosome 1 (p62/SQSTM1) are among the most widely used markers to monitor autophagy and can be visualized in formalin-fixed and paraffin-embedded tissue by immunohistochemistry. Here we describe a validated staining protocol using an automated staining system available in many routine pathology laboratories, enabling high-throughput staining under standardized conditions.
Item Type: |
Journal Article (Further Contribution) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > Service Sector > Institute of Pathology 04 Faculty of Medicine > Service Sector > Institute of Pathology > Clinical Pathology |
UniBE Contributor: |
Berezowska, Sabina Anna, Galván Hernández, José Alberto |
Subjects: |
500 Science > 570 Life sciences; biology 600 Technology > 610 Medicine & health |
ISSN: |
1940-6029 |
Publisher: |
Springer |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
26 Sep 2022 08:38 |
Last Modified: |
05 Dec 2022 16:25 |
Publisher DOI: |
10.1007/978-1-0716-2675-7_10 |
PubMed ID: |
36152247 |
Uncontrolled Keywords: |
Autophagy Immunohistochemistry LC3 Sequestosome 1 p62/SQSTM1 |
BORIS DOI: |
10.48350/173257 |
URI: |
https://boris.unibe.ch/id/eprint/173257 |
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