Single p197 molecules of the mitochondrial genome segregation system of Trypanosoma brucei determine the distance between basal body and outer membrane.

Aeschlimann, Salome; Kalichava, Ana; Schimanski, Bernd; Berger, Bianca Manuela; Jetishi, Clirim; Stettler, Philip; Ochsenreiter, Torsten; Schneider, André (2022). Single p197 molecules of the mitochondrial genome segregation system of Trypanosoma brucei determine the distance between basal body and outer membrane. Proceedings of the National Academy of Sciences of the United States of America - PNAS, 119(40), e2204294119. National Academy of Sciences NAS 10.1073/pnas.2204294119

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The tripartite attachment complex (TAC) couples the segregation of the single unit mitochondrial DNA of trypanosomes with the basal body (BB) of the flagellum. Here, we studied the architecture of the exclusion zone filament (EZF) of the TAC, the only known component of which is p197, that connects the BB with the mitochondrial outer membrane (OM). We show that p197 has three domains that are all essential for mitochondrial DNA inheritance. The C terminus of p197 interacts with the mature and probasal body (pro-BB), whereas its N terminus binds to the peripheral OM protein TAC65. The large central region of p197 has a high α-helical content and likely acts as a flexible spacer. Ultrastructure expansion microscopy (U-ExM) of cell lines exclusively expressing p197 versions of different lengths that contain both N- and C-terminal epitope tags demonstrates that full-length p197 alone can bridge the ∼270-nm distance between the BB and the cytosolic face of the OM. Thus U-ExM allows the localization of distinct domains within the same molecules and suggests that p197 is the TAC subunit most proximal to the BB. In addition, U-ExM revealed that p197 acts as a spacer molecule, as two shorter versions of p197, with the repeat domain either removed or replaced by the central domain of the Trypanosoma cruzi p197 ortholog reduced the distance between the BB and the OM in proportion to their predicted molecular weight.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Chemistry, Biochemistry and Pharmaceutical Sciences (DCBP)
08 Faculty of Science > Department of Biology > Institute of Cell Biology

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Aeschlimann, Salome Johanna; Kalichava, Ana; Schimanski, Bernd; Berger, Bianca Manuela; Jetishi, Clirim; Stettler, Philip; Ochsenreiter, Torsten and Schneider, André

Subjects:

500 Science > 570 Life sciences; biology
500 Science > 540 Chemistry

ISSN:

0027-8424

Publisher:

National Academy of Sciences NAS

Language:

English

Submitter:

Pubmed Import

Date Deposited:

29 Sep 2022 10:56

Last Modified:

05 Dec 2022 16:25

Publisher DOI:

10.1073/pnas.2204294119

PubMed ID:

36161893

Uncontrolled Keywords:

Trypanosoma basal body genome segregation kinetoplast DNA mitochondrial genome

BORIS DOI:

10.48350/173355

URI:

https://boris.unibe.ch/id/eprint/173355

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