Prediction of Biochemical Recurrence Based on Molecular Detection of Lymph Node Metastasis After Radical Prostatectomy.

Özdemir, Berna C; Arnold, Nicolas; Fleischmann, Achim; Hensel, Janine; Klima, Irena; Kruithof-de Julio, Marianna; Burkhard, Fiona; Hayoz, Stefanie; Kiss, Bernhard; Thalmann, George N (2022). Prediction of Biochemical Recurrence Based on Molecular Detection of Lymph Node Metastasis After Radical Prostatectomy. European urology open science, 44, pp. 1-10. Elsevier 10.1016/j.euros.2022.07.005

[img]
Preview
Text
1-s2.0-S266616832200739X-main.pdf - Published Version
Available under License Creative Commons: Attribution (CC-BY).

Download (2MB) | Preview

Background

Molecular detection of lymph node (LN) micrometastases by analyzing mRNA expression of epithelial markers in prostate cancer (PC) patients provides higher sensitivity than histopathological examination.

Objective

To investigate which type of marker to use and whether molecular detection of micrometastases in LNs was predictive of biochemical recurrence.

Design setting and participants

LN samples from PC patients undergoing radical prostatectomy with extended LN dissection between 2009 and 2011 were examined for the presence of micrometastases by both routine histopathology and molecular analyses.

Outcome measurements and statistical analysis

The mRNA expression of a panel of markers of prostate epithelial cells, prostate stem cell-like cells, epithelial-to-mesenchymal transition, and stromal activation, was performed by quantitative real-time polymerase chain reaction. The expression levels of these markers in LN metastases from three PC patients were compared with the expression levels in LN from five control patients without PC in order to identify the panel of markers best suited for the molecular detection of LN metastases. The predictive value of the molecular detection of micrometastases for biochemical recurrence was assessed after a follow-up of 10 yr.

Results and limitations

Prostate epithelial markers are better suited for the detection of occult LN metastases than molecular markers of stemness, epithelial-to-mesenchymal transition, or reactive stroma. An analysis of 1023 LNs from 60 PC patients for the expression of prostate epithelial cell markers has revealed different expression levels and patterns between patients and between LNs of the same patient. The positive predictive value of molecular detection of occult LN metastasis for biochemical recurrence is 66.7% and the negative predictive value is 62.5%. Limitations are sample size and the hypothesis-driven selection of markers.

Conclusions

Molecular detection of epithelial cell markers increases the number of positive LNs and predicts tumor recurrence already at surgery.

Patient summary

We show that a panel of epithelial prostate markers rather than single genes is preferred for the molecular detection of lymph node micrometastases not visible at histopathological examination.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Urologie
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Urologie

04 Faculty of Medicine > Department of Dermatology, Urology, Rheumatology, Nephrology, Osteoporosis (DURN) > Clinic of Urology
04 Faculty of Medicine > Service Sector > Institute of Pathology
04 Faculty of Medicine > Department of Haematology, Oncology, Infectious Diseases, Laboratory Medicine and Hospital Pharmacy (DOLS) > Clinic of Medical Oncology

UniBE Contributor:

Özdemir, Berna; Arnold, Nicolas; Fleischmann, Achim; Hensel, Janine; Klima, Irena; Kruithof-de Julio, Marianna; Burkhard, Fiona Christine; Kiss, Bernhard and Thalmann, George

Subjects:

600 Technology > 610 Medicine & health
500 Science > 570 Life sciences; biology

ISSN:

2666-1683

Publisher:

Elsevier

Language:

English

Submitter:

Pubmed Import

Date Deposited:

04 Oct 2022 14:49

Last Modified:

05 Dec 2022 16:25

Publisher DOI:

10.1016/j.euros.2022.07.005

PubMed ID:

36185585

Uncontrolled Keywords:

Extended lymphadenectomy Isolated tumor cells Lymph nodes Metastasis Micrometastasis Molecular detection Molecular markers Prostate cancer mRNA expression

BORIS DOI:

10.48350/173477

URI:

https://boris.unibe.ch/id/eprint/173477

Actions (login required)

Edit item Edit item
Provide Feedback