Bayer, Julia; Stroka, Deborah; Kubes, Paul; Candinas, Daniel; Zindel, Joel (2022). Combination of Sterile Injury and Microbial Contamination to Model Post-surgical Peritoneal Adhesions in Mice. Bio-protocol, 12(16) 10.21769/BioProtoc.4491
Full text not available from this repository.Abdominal surgeries are frequently associated with the development of post-surgical adhesions. These are irreversible fibrotic scar bands that appear between abdominal organs and the abdominal wall. Patients suffering from adhesions are at risk of severe complications, such as small bowel obstruction, chronic pelvic pain, or infertility. To date, no cure exists, and the understanding of underlying molecular mechanisms of adhesion formation is incomplete. The current paradigm largely relies on sterile injury mouse models. However, abdominal surgeries in human patients are rarely completely sterile procedures. Here, we describe a modular surgical procedure for simultaneous or separate induction of sterile injury and microbial contamination. Combined, these insults synergistically lead to adhesion formation in the mouse peritoneal cavity. Surgical trauma is confined to a localized sterile injury of the peritoneum. Microbial contamination of the peritoneal cavity is induced by a limited perforation of the microbe-rich large intestine or by injection of fecal content. The presented protocol extends previous injury-based adhesion models by an additional insult through microbial contamination, which may more adequately model the clinical context of abdominal surgery. Graphical abstract.
Item Type: |
Journal Article (Further Contribution) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Visceral Surgery and Medicine > Visceral Surgery 04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Visceral Surgery and Medicine |
Graduate School: |
Graduate School for Cellular and Biomedical Sciences (GCB) |
UniBE Contributor: |
Bayer, Julia Isabella, Stroka, Deborah, Candinas, Daniel, Zindel, Joel |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
2331-8325 |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
07 Oct 2022 09:47 |
Last Modified: |
05 Dec 2022 16:26 |
Publisher DOI: |
10.21769/BioProtoc.4491 |
PubMed ID: |
36199704 |
Uncontrolled Keywords: |
Cecal ligation and puncture Mice Microbe contamination Peritoneal adhesion index Peritoneal button Peritoneum Post-surgical adhesion Surgery |
URI: |
https://boris.unibe.ch/id/eprint/173563 |
Actions (login required)
 |
Edit item |