Birrer, Fabienne; Brodie, Tess; Stroka, Deborah (2023). OMIP-088: Twenty-target imaging mass cytometry panel for major cell populations in mouse formalin fixed paraffin embedded liver. Cytometry Part A, 103(3), pp. 189-192. Wiley 10.1002/cyto.a.24714
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Cytometry_Pt_A_-_2023_-_Birrer_-_OMIP_088_Twenty_target_imaging_mass_cytometry_panel_for_major_cell_populations_in_mouse.pdf - Published Version Restricted to registered users only Available under License Publisher holds Copyright. Download (1MB) |
The purpose of this 20-target imaging mass cytometry (IMC) panel is to identify the main cell types in formalin fixed paraffin embedded (FFPE) mouse liver tissue with the Hyperion™ mass cytometer from Standard BioTools (formerly Fluidigm). The antibody panel includes markers to identify hepatocytes (E-cadherin, HNF4α (hepatocyte nuclear factor 4 alpha), Arginase-1), liver sinusoidal endothelial cells (LSECs; CD206), Kupffer cells (F4/80, CD206), neutrophils (Ly6G, CD11b), bone marrow derived myeloid cells (BMDMs; CD11b), cholangiocytes (E-cadherin high), endothelial cells (CD31, α-SMA), plasmacytoid dendritic cells (CD317), B cells (CD19), T cells (CD3e, CD4, CD8a), NK cells (CD161) as well markers of cell activation (CD44, CD74), proliferation (Ki-67) and to aid in cell segmentation (Pan-Actin, E-cadherin, histone H3). The panel has been tested in other mouse tissues, namely the spleen, colon and lung, and therefore is likely to work across various mouse FFPE samples of interest. It has not been tested using human samples, frozen samples or in suspension mass cytometry because FFPE treatment profoundly changes epitope conformation. In summary, this panel is a powerful tool for pre-clinical research to determine cellular abundance and spatial distribution within mouse tissues and serves as a scaffold, to which more targets can be added for project specific requirements.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
09 Interdisciplinary Units > Microscopy Imaging Center (MIC) 04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Visceral Surgery and Medicine > Visceral Surgery |
Graduate School: |
Graduate School for Cellular and Biomedical Sciences (GCB) |
UniBE Contributor: |
Birrer, Fabienne Esther, Brodie, Tess Melinda, Stroka, Deborah |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
1552-4930 |
Publisher: |
Wiley |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
06 Jan 2023 10:47 |
Last Modified: |
21 Jun 2024 10:19 |
Publisher DOI: |
10.1002/cyto.a.24714 |
PubMed ID: |
36602064 |
Uncontrolled Keywords: |
cell segmentation imaging mass cytometry metal labeled antibodies mouse FFPE tissues panel design panel validation |
BORIS DOI: |
10.48350/176902 |
URI: |
https://boris.unibe.ch/id/eprint/176902 |
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