Coatsome-replicon vehicles: Self-replicating RNA vaccines against infectious diseases.

Démoulins, Thomas; Schulze, Kai; Ebensen, Thomas; Techakriengkrai, Navapon; Nedumpun, Teerawut; Englezou, Pavlos C; Gerber, Markus; Hlushchuk, Ruslan; Toledo, Darien; Djonov, Valentin; von Gunten, Stephan; McCullough, Kenneth C; Liniger, Matthias; Guzmán, Carlos A; Suradhat, Sanipa; Ruggli, Nicolas (2023). Coatsome-replicon vehicles: Self-replicating RNA vaccines against infectious diseases. Nanomedicine : nanotechnology, biology, and medicine, 49(102655), p. 102655. Elsevier 10.1016/j.nano.2023.102655

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Herein, we provide the first description of a synthetic delivery method for self-replicating replicon RNAs (RepRNA) derived from classical swine fever virus (CSFV) using a Coatsome-replicon vehicle based on Coatsome® SS technologies. This results in an unprecedented efficacy when compared to well-established polyplexes, with up to ~65 fold-increase of the synthesis of RepRNA-encoded gene of interest (GOI). We demonstrated the efficacy of such Coatsome-replicon vehicles for RepRNA-mediated induction of CD8 T-cell responses in mice. Moreover, we provide new insights on physical properties of the RepRNA, showing that the removal of all CSFV structural protein genes has a positive effect on the translation of the GOI. Finally, we successfully engineered RepRNA constructs encoding a porcine reproductive and respiratory syndrome virus (PRRSV) antigen, providing an example of antigen expression with potential application to combat viral diseases. The versatility and simplicity of modifying and manufacturing these Coatsome-replicon vehicle formulations represents a major asset to tackle foreseeable emerging pandemics.

Item Type:

Journal Article (Original Article)


04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Pharmacology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Virology and Immunology

UniBE Contributor:

Démoulins, Thomas Paul Rémi, Englezou, Pavlos, Gerber, Markus Daniel, Hlushchuk, Ruslan, Toledo Santamaria, Darien, Djonov, Valentin Georgiev, von Gunten, Stephan, McCullough, Kenneth, Liniger, Matthias, Ruggli, Nicolas


600 Technology > 630 Agriculture
500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health








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Date Deposited:

26 Jan 2023 13:34

Last Modified:

19 Jan 2024 00:25

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Uncontrolled Keywords:

Coatsome-replicon vehicle Porcine reproductive and respiratory syndrome virus (PRRSV) Self-amplifying replicon RNA (RepRNA) Structural protein genes c-di-AMP




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