Haddad, Tariq Sami; van den Dobbelsteen, Luuk; Öztürk, Sonay K; Geene, Robin; Nijman, Isaäc J; Verrijp, Kiek; Jamieson, Nigel B; Wood, Colin; van Vliet, Shannon; Reuvers, Luuk; Achouiti, Soumia; Rutgers, Natasja; Brouwer, Nelleke; Simmer, Femke; Zlobec, Inti; Lugli, Alessandro; Nagtegaal, Iris D (2023). Pseudobudding: ruptured glands do not represent true tumor buds. The journal of pathology: clinical research, 261(1), pp. 19-27. Wiley 10.1002/path.6146
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The_Journal_of_Pathology_-_2023_-_Haddad_-_Pseudobudding_ruptured_glands_do_not_represent_true_tumor_buds.pdf - Published Version Available under License Creative Commons: Attribution (CC-BY). Download (84MB) | Preview |
Tumor budding (TB) is a strong biomarker of poor prognosis in colorectal cancer and other solid cancers. TB is defined as isolated single cancer cells or clusters of up to four cancer cells at the invasive tumor front. In areas with a large inflammatory response at the invasive front, single cells and cell clusters surrounding fragmented glands are observed appearing like TB. Occurrence of these small groups is referred to as pseudobudding (PsB), which arises due to external influences such as inflammation and glandular disruption. Using a combination of orthogonal approaches, we show that there are clear biological differences between TB and PsB. TB is representative of active invasion by presenting features of epithelial-mesenchymal transition and exhibiting increased deposition of extracellular matrix within the surrounding tumor microenvironment (TME), whereas PsB represents a reactive response to heavy inflammation where increased levels of granulocytes within the surrounding TME are observed. Our study provides evidence that areas with a strong inflammatory reaction should be avoided in the routine diagnostic assessment of TB. © 2023 The Authors. The Journal of Pathology published by John Wiley & Sons Ltd on behalf of The Pathological Society of Great Britain and Ireland.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
04 Faculty of Medicine > Service Sector > Institute of Pathology > Clinical Pathology 04 Faculty of Medicine > Service Sector > Institute of Pathology |
UniBE Contributor: |
Zlobec, Inti, Lugli, Alessandro |
Subjects: |
600 Technology > 610 Medicine & health 500 Science > 570 Life sciences; biology |
ISSN: |
2056-4538 |
Publisher: |
Wiley |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
05 Jul 2023 15:48 |
Last Modified: |
31 Aug 2023 00:15 |
Publisher DOI: |
10.1002/path.6146 |
PubMed ID: |
37403270 |
Uncontrolled Keywords: |
colorectal cancer immunohistochemistry multiplex immunofluorescence pseudobudding spatial transcriptomics transmission electron microscopy tumor budding |
BORIS DOI: |
10.48350/184513 |
URI: |
https://boris.unibe.ch/id/eprint/184513 |
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