Kalichava, Ana; Ochsenreiter, Torsten (2021). Ultrastructure expansion microscopy in Trypanosoma brucei. Open Biology, 11(10), p. 210132. The Royal Society Publishing 10.1098/rsob.210132
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The recently developed ultrastructure expansion microscopy (U-ExM) technique allows us to increase the spatial resolution within a cell or tissue for microscopic imaging through the physical expansion of the sample. In this study, we validate the use of U-ExM in Trypanosoma brucei measuring the expansion factors of several different compartments/organelles and thus verify the isotropic expansion of the cell. We furthermore demonstrate the use of this sample preparation protocol for future studies by visualizing the nucleus and kDNA, as well as proteins of the cytoskeleton, the basal body, the mitochondrion and the endoplasmic reticulum. Lastly, we discuss the challenges and opportunities of U-ExM.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
08 Faculty of Science > Department of Biology > Institute of Cell Biology 08 Faculty of Science > Department of Biology > Institute of Cell Biology > Mitoch. |
Graduate School: |
Graduate School for Cellular and Biomedical Sciences (GCB) |
UniBE Contributor: |
Kalichava, Ana, Ochsenreiter, Torsten |
Subjects: |
500 Science > 570 Life sciences; biology |
ISSN: |
2046-2441 |
Publisher: |
The Royal Society Publishing |
Language: |
English |
Submitter: |
Torsten Ochsenreiter |
Date Deposited: |
19 Jul 2023 09:57 |
Last Modified: |
19 Jul 2023 09:57 |
Publisher DOI: |
10.1098/rsob.210132 |
PubMed ID: |
34637654 |
Uncontrolled Keywords: |
T. brucei cell structure centriole expansion microscopy kDNA super resolution |
BORIS DOI: |
10.48350/184832 |
URI: |
https://boris.unibe.ch/id/eprint/184832 |
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