Cooper, Sinclair; Wadsworth, Elizabeth S; Ochsenreiter, Torsten; Ivens, Alasdair; Savill, Nicholas J; Schnaufer, Achim (2019). Assembly and annotation of the mitochondrial minicircle genome of a differentiation-competent strain of Trypanosoma brucei. Nucleic acids research, 47(21), pp. 11304-11325. Oxford University Press 10.1093/nar/gkz928
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Kinetoplastids are protists defined by one of the most complex mitochondrial genomes in nature, the kinetoplast. In the sleeping sickness parasite Trypanosoma brucei, the kinetoplast is a chain mail-like network of two types of interlocked DNA molecules: a few dozen ∼23-kb maxicircles (homologs of the mitochondrial genome of other eukaryotes) and thousands of ∼1-kb minicircles. Maxicircles encode components of respiratory chain complexes and the mitoribosome. Several maxicircle-encoded mRNAs undergo extensive post-transcriptional RNA editing via addition and deletion of uridines. The process is mediated by hundreds of species of minicircle-encoded guide RNAs (gRNAs), but the precise number of minicircle classes and gRNA genes was unknown. Here we present the first essentially complete assembly and annotation of the kinetoplast genome of T. brucei. We have identified 391 minicircles, encoding not only ∼930 predicted 'canonical' gRNA genes that cover nearly all known editing events (accessible via the web at http://hank.bio.ed.ac.uk), but also ∼370 'non-canonical' gRNA genes of unknown function. Small RNA transcriptome data confirmed expression of the majority of both categories of gRNAs. Finally, we have used our data set to refine definitions for minicircle structure and to explore dynamics of minicircle copy numbers.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
08 Faculty of Science > Department of Biology > Institute of Cell Biology 08 Faculty of Science > Department of Biology > Institute of Cell Biology > Mitoch. |
Graduate School: |
Graduate School for Cellular and Biomedical Sciences (GCB) |
UniBE Contributor: |
Ochsenreiter, Torsten |
Subjects: |
500 Science > 570 Life sciences; biology |
ISSN: |
1362-4962 |
Publisher: |
Oxford University Press |
Language: |
English |
Submitter: |
Torsten Ochsenreiter |
Date Deposited: |
19 Jul 2023 10:00 |
Last Modified: |
01 Apr 2024 22:54 |
Publisher DOI: |
10.1093/nar/gkz928 |
PubMed ID: |
31665448 |
BORIS DOI: |
10.48350/184835 |
URI: |
https://boris.unibe.ch/id/eprint/184835 |
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