Mobilization in two-step capillary isoelectric focusing: Concepts assessed by computer simulation.

Thormann, Wolfgang; Mosher, Richard A (2024). Mobilization in two-step capillary isoelectric focusing: Concepts assessed by computer simulation. Electrophoresis, 45(7-8), pp. 618-638. Wiley-VCH 10.1002/elps.202300218

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The mobilization step in a two-step capillary isoelectric focusing protocol is discussed by means of dynamic computer simulation data for systems without and with spacer compounds that establish their zones at the beginning and end of the focusing column. After focusing in an electroosmosis-free environment (first step), mobilization (second step) can be induced electrophoretically, by the application of a hydrodynamic flow, or by a combination of both means. Dynamic simulations provide insight into the complexity of the various modes of electrophoretic mobilization and dispersion associated with hydrodynamic mobilization. The data are discussed together with the relevant literature.

Item Type:

Journal Article (Review Article)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases

UniBE Contributor:

Thormann, Wolfgang

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

0173-0835

Publisher:

Wiley-VCH

Language:

English

Submitter:

Pubmed Import

Date Deposited:

21 Dec 2023 11:12

Last Modified:

28 Apr 2024 00:13

Publisher DOI:

10.1002/elps.202300218

PubMed ID:

38115749

Uncontrolled Keywords:

capillary isoelectric focusing chemical mobilization computer simulation electrophoretic mobilization hydrodynamic mobilization

BORIS DOI:

10.48350/190591

URI:

https://boris.unibe.ch/id/eprint/190591

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