Thormann, Wolfgang; Mosher, Richard A (2024). Mobilization in two-step capillary isoelectric focusing: Concepts assessed by computer simulation. Electrophoresis, 45(7-8), pp. 618-638. Wiley-VCH 10.1002/elps.202300218
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Electrophoresis_-_2023_-_Thormann_-_Mobilization_in_two_step_capillary_isoelectric_focusing__Concepts_assessed_by_computer.pdf - Published Version Restricted to registered users only Available under License Publisher holds Copyright. Download (8MB) |
The mobilization step in a two-step capillary isoelectric focusing protocol is discussed by means of dynamic computer simulation data for systems without and with spacer compounds that establish their zones at the beginning and end of the focusing column. After focusing in an electroosmosis-free environment (first step), mobilization (second step) can be induced electrophoretically, by the application of a hydrodynamic flow, or by a combination of both means. Dynamic simulations provide insight into the complexity of the various modes of electrophoretic mobilization and dispersion associated with hydrodynamic mobilization. The data are discussed together with the relevant literature.
Item Type: |
Journal Article (Review Article) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases |
UniBE Contributor: |
Thormann, Wolfgang |
Subjects: |
500 Science > 570 Life sciences; biology 600 Technology > 610 Medicine & health |
ISSN: |
0173-0835 |
Publisher: |
Wiley-VCH |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
21 Dec 2023 11:12 |
Last Modified: |
28 Apr 2024 00:13 |
Publisher DOI: |
10.1002/elps.202300218 |
PubMed ID: |
38115749 |
Uncontrolled Keywords: |
capillary isoelectric focusing chemical mobilization computer simulation electrophoretic mobilization hydrodynamic mobilization |
BORIS DOI: |
10.48350/190591 |
URI: |
https://boris.unibe.ch/id/eprint/190591 |
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