Mobilization in two-step capillary isoelectric focusing: Concepts assessed by computer simulation.

Thormann, Wolfgang; Mosher, Richard A (2024). Mobilization in two-step capillary isoelectric focusing: Concepts assessed by computer simulation. Electrophoresis, 45(7-8), pp. 618-638. Wiley-VCH 10.1002/elps.202300218

Text Electrophoresis_-_2023_-_Thormann_-_Mobilization_in_two_step_capillary_isoelectric_focusing__Concepts_assessed_by_computer.pdf - Published Version Restricted to registered users only Available under License Publisher holds Copyright. Download (8MB) | Request a copy

The mobilization step in a two-step capillary isoelectric focusing protocol is discussed by means of dynamic computer simulation data for systems without and with spacer compounds that establish their zones at the beginning and end of the focusing column. After focusing in an electroosmosis-free environment (first step), mobilization (second step) can be induced electrophoretically, by the application of a hydrodynamic flow, or by a combination of both means. Dynamic simulations provide insight into the complexity of the various modes of electrophoretic mobilization and dispersion associated with hydrodynamic mobilization. The data are discussed together with the relevant literature.

Interest & Impact

Downloads

0 since deposited on 21 Dec 2023
0 in the past 12 months

Citations

5 Citations in Web of Science ®
6 Citations in Scopus

Search

in Google Scholar™

Services

Actions (login required)

Edit item

Actions (login required)

Edit item