Active caspase 3 and DNA fragmentation as markers for apoptotic cell death in primary and metastatic liver tumours

Karamitopoulou, Eva; Cioccari, Luca; Jakob, Sabine; Vallan, Claudio; Schaffner, Thomas; Zimmermann, Arthur; Brunner, Thomas (2007). Active caspase 3 and DNA fragmentation as markers for apoptotic cell death in primary and metastatic liver tumours. Pathology, 39(6), pp. 558-64. London: Wolters Kluwer Lippincott Williams & Wilkins 10.1080/00313020701684375

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AIMS: The induction of tumour cell death by apoptosis is a major goal of cancer therapy and the in situ detection of apoptosis in tumour tissue has become an important diagnostic parameter. Different apoptosis detection methods assess distinct biochemical processes in the dying cell. Thus, their direct comparison is mandatory to evaluate their diagnostic value. The aim of this study was to compare the immunohistochemical detection of active caspase 3 and single-stranded DNA in primary and metastatic liver tumours as markers of apoptotic cell death. METHODS: We studied detection of active caspase 3 and single-stranded DNA in 20 primary hepatocellular carcinomas (HCC) and 20 liver metastases from colorectal carcinomas (CRC) using immunohistochemistry on paraffin sections. RESULTS: Our results reveal that both methods are suitable and sensitive techniques for the in situ detection of apoptosis, however, they also demonstrate that immunohistochemistry for active caspase 3 and single-stranded DNA have differential sensitivities in HCC and CRC. CONCLUSION: The sensitivity of apoptosis detection using immunohistochemistry for active caspase 3 and single-stranded DNA may be tumour cell type dependent.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute of Pathology

UniBE Contributor:

Schaffner, Thomas; Zimmermann, Arthur and Brunner, Thomas

ISSN:

0031-3025

ISBN:

18027258

Publisher:

Wolters Kluwer Lippincott Williams & Wilkins

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:53

Last Modified:

04 May 2014 23:15

Publisher DOI:

10.1080/00313020701684375

PubMed ID:

18027258

Web of Science ID:

000251931500004

URI:

https://boris.unibe.ch/id/eprint/22675 (FactScience: 35918)

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