The promiscuous role of the epsilon subunit in GABA(A) receptor biogenesis

Bollan, Karen A; Baur, Roland; Hales, Tim G; Sigel, Erwin; Connolly, Christopher N (2008). The promiscuous role of the epsilon subunit in GABA(A) receptor biogenesis. Molecular and cellular neuroscience, 37(3), pp. 610-621. San Diego, Calif.: Elsevier 10.1016/j.mcn.2007.12.011

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The formation of alpha1beta2gamma2epsilon receptors suggests that the epsilon subunit does not displace the single gamma2 subunit in alpha1beta2gamma2 receptors. Thus, epsilon must replace alpha and/or beta subunit(s) if the pentameric receptor structure is to be preserved. To assess the potential for which subunit is replaced in alphabetaepsilon and alphabetagammaepsilon receptors we analyzed the assembly and functional expression of the epsilon subunit with respect to alpha1, beta2 and gamma2 subunits. Using concatenated subunits, we have determined that epsilon is capable of substituting for either (but not both) of the alpha subunits, one of the beta subunits, and possibly the gamma2 subunit. However, the most likely sites at which the epsilon subunit may contribute to receptor function appears to be at position 1 (replaces alpha1) in alphabetagammaepsilon (varepsilon-beta2-alpha1-beta2-gamma2) receptors, or at position 4 (replaces beta2) in alphabetaepsilon (alpha1-beta2-alpha1-varepsilon-beta2) receptors. In both cases, it appears that only a single GABA binding site is present.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Biochemistry and Molecular Medicine

UniBE Contributor:

Sigel, Erwin

ISSN:

1044-7431

ISBN:

18206389

Publisher:

Elsevier

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:53

Last Modified:

08 Jun 2016 10:42

Publisher DOI:

10.1016/j.mcn.2007.12.011

PubMed ID:

18206389

Web of Science ID:

000253928500018

URI:

https://boris.unibe.ch/id/eprint/22706 (FactScience: 36102)

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