The promiscuous role of the epsilon subunit in GABA(A) receptor biogenesis

Bollan, Karen A; Baur, Roland; Hales, Tim G; Sigel, Erwin; Connolly, Christopher N (2008). The promiscuous role of the epsilon subunit in GABA(A) receptor biogenesis. Molecular and cellular neuroscience, 37(3), pp. 610-621. San Diego, Calif.: Elsevier 10.1016/j.mcn.2007.12.011

Full text not available from this repository.

The formation of alpha1beta2gamma2epsilon receptors suggests that the epsilon subunit does not displace the single gamma2 subunit in alpha1beta2gamma2 receptors. Thus, epsilon must replace alpha and/or beta subunit(s) if the pentameric receptor structure is to be preserved. To assess the potential for which subunit is replaced in alphabetaepsilon and alphabetagammaepsilon receptors we analyzed the assembly and functional expression of the epsilon subunit with respect to alpha1, beta2 and gamma2 subunits. Using concatenated subunits, we have determined that epsilon is capable of substituting for either (but not both) of the alpha subunits, one of the beta subunits, and possibly the gamma2 subunit. However, the most likely sites at which the epsilon subunit may contribute to receptor function appears to be at position 1 (replaces alpha1) in alphabetagammaepsilon (varepsilon-beta2-alpha1-beta2-gamma2) receptors, or at position 4 (replaces beta2) in alphabetaepsilon (alpha1-beta2-alpha1-varepsilon-beta2) receptors. In both cases, it appears that only a single GABA binding site is present.

Item Type:	Journal Article (Original Article)
Division/Institute:	04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Biochemistry and Molecular Medicine
UniBE Contributor:	Sigel, Erwin
ISSN:	1044-7431
ISBN:	18206389
Publisher:	Elsevier
Language:	English
Submitter:	Factscience Import
Date Deposited:	04 Oct 2013 14:53
Last Modified:	05 Dec 2022 14:16
Publisher DOI:	10.1016/j.mcn.2007.12.011
PubMed ID:	18206389
Web of Science ID:	000253928500018
URI:	https://boris.unibe.ch/id/eprint/22706 (FactScience: 36102)