Detection of apoptosis in vivo using antibodies against caspase-induced neo-epitopes

Jakob, Sabine; Corazza, Nadia; Diamantis, Eva; Kappeler, Andreas; Brunner, Thomas (2008). Detection of apoptosis in vivo using antibodies against caspase-induced neo-epitopes. Methods, 44(3), pp. 255-61. San Diego, Calif.: Elsevier 10.1016/j.ymeth.2007.11.004

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Cell death induction by apoptosis is an important process in the maintenance of tissue homeostasis as well as tissue destruction during various pathological processes. Consequently, detection of apoptotic cells in situ represents an important technique to assess the extent and impact of cell death in the respective tissue. While scoring of apoptosis by histological assessment of apoptotic cells is still a widely used method, it is likely biased by sensitivity problems and observed-based variations. The availability of caspase-mediated neo-epitope-specific antibodies offers new tools for the detection of apoptosis in situ. Here, we discuss the use of immunohistochemical detection of cleaved caspase 3 and lamin A for the assessment of apoptotic cells in paraffin-embedded liver tissue. Furthermore, we evaluate the effect of tissue pretreatment and antigen retrieval on the sensitivity of apoptosis detection, background staining and maintenance of tissue morphology.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute of Pathology
04 Faculty of Medicine > Service Sector > Institute of Pathology > Immunopathology

UniBE Contributor:

Schneider, Sabine; Corazza, Nadia; Kappeler, Andreas and Brunner, Thomas

ISSN:

1046-2023

ISBN:

18314057

Publisher:

Elsevier

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 15:02

Last Modified:

04 May 2014 23:19

Publisher DOI:

10.1016/j.ymeth.2007.11.004

PubMed ID:

18314057

Web of Science ID:

000254689300009

URI:

https://boris.unibe.ch/id/eprint/27076 (FactScience: 101687)

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